Purification and characterization of two types of atrial natriuretic factor receptors from bovine adrenal cortex: guanylate cyclase-linked and cyclase-free receptors.
Purification and characterization of two types of atrial natriuretic factor receptors from bovine adrenal cortex: guanylate cyclase-linked and cyclase-free receptors.
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来自牛肾上腺皮质的两种类型的心房钠尿因子受体的纯化和表征：鸟苷酸环化酶连接的受体和无环化酶的受体。
DOI：
10.1016/s0006-291x(87)80502-8
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发表时间：
1987
影响因子：
3.1
通讯作者：
Inagami,T
中科院分区：
文献类型：
--
作者：
Takayanagi,R;Snajdar,RM;Imada,T;Tamura,M;Pandey,KN;Misono,KS;Inagami,T
从牛肾上腺髓质肾小球细胞膜组分中同时纯化具有和不具有鸟苷酸环化酶活性的心房利钠因子（ANF）受体，使其具有明显的均一性。与ANF受体共纯化的颗粒鸟苷酸环化酶显示出最高的比活性之一。具有或不具有鸟苷酸环化酶活性的受体对ANF（99-126）表现出高亲和力。没有环化酶的受体显示出对截短的ANF类似物ANF（103-123）和ANF（105-121）的高亲和力，而环化酶连接的受体对这些类似物具有低得多的亲和力。在非还原条件下，两种受体在SDS-凝胶电泳上以分子量为135，000道尔顿的单一条带迁移。在还原条件下，不含环化酶的受体的135，000道尔顿条带移动到62，000道尔顿条带，但环化酶连接的受体的条带没有移动。这些结果表明牛肾上腺皮质中存在两种亚型的ANF受体，并表明ANF的两种不同的细胞内作用模式。
Atrial natriuretic factor(ANF) receptors with and without guanylate cyclase activity were simultaneously purified to apparent homogeneity from bovine adrenal zona glomerulosa cell membrane fractions. The particulate guanylate cyclase which co-purified with the ANF receptor showed one of the highest specific activity reported. The receptors with or without the guanylate cyclase activity showed high affinities to ANF(99–126). The receptor without the cyclase showed a high affinity to truncated ANF analogs, ANF(103–123)and ANF(105–121), whereas the cyclase-linked receptor had a much lower affinity to these analogs. Both of the receptors migrated as a single band with a molecular weight of 135,000 daltons on SDS-gel electrophoresis under non-reducing conditions. The 135,000 daltons band of the receptor without the cyclase was shifted to a 62,000 daltons band under reducing conditions, but the band for the cyclase-linked receptor was not shifted. These results demonstrated the presence of two subtypes of ANF receptor in bovine adrenal cortex and indicate two different modes of intracellular action of ANF.