POSITIONALLY INDEPENDENT AND EXCHANGEABLE LATE BUDDING FUNCTIONS OF THE ROUS-SARCOMA VIRUS AND HUMAN-IMMUNODEFICIENCY-VIRUS GAG PROTEINS
POSITIONALLY INDEPENDENT AND EXCHANGEABLE LATE BUDDING FUNCTIONS OF THE ROUS-SARCOMA VIRUS AND HUMAN-IMMUNODEFICIENCY-VIRUS GAG PROTEINS
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DOI：
10.1128/jvi.69.9.5455-5460.1995
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发表时间：
1995-09-01
影响因子：
5.4
通讯作者：
WILLS, JW
中科院分区：
文献类型：
--
作者：
PARENT, LJ;BENNETT, RP;WILLS, JW
劳斯肉瘤病毒和人类免疫缺陷病毒（HIV）的Gag蛋白各自含有涉及出芽的后期步骤的功能，其中缺陷导致这些分子在质膜上的积累。在劳斯肉瘤病毒Gag蛋白（pr76（gag））中，该组装结构域与PPPY基序相关，该基序位于MA和CA序列之间的内部位置。该基序不包含在HIV Gag蛋白（pr55（gag））内的任何地方，并且MA序列直接连接到CA。相反，HIV的晚期组装功能与位于Gag C末端的p6序列相关。在这里，我们证明了显着的发现，从这两个不相关的Gag蛋白的后期组装域之间的逆转录病毒是可交换的，可以在一个位置独立的方式发挥作用。
The Gag proteins of Rous sarcoma virus and human immunodeficiency virus (HIV) each contain a function involved in a late step in budding, defects in which result in the accumulation of these molecules at the plasma membrane. In the Rous sarcoma virus Gag protein (pr76(gag)), this assembly domain is associated with a PPPY motif, which is located at an internal position between the MA and CA sequences. This motif is not contained anywhere within the HIV Gag protein (pr55(gag)), and the MA sequence is linked directly to CA. Instead, a late assembly function of HIV has been associated with the p6 sequence situated at the C terminus of Gag. Here we demonstrate the remarkable finding that the late assembly domains from these two unrelated Gag proteins are exchangeable between retroviruses and can function in a positionally independent manner.