Regional molecular genetic key of thirteen snow pool Aedes species (Diptera:Culicidae) in northern Colorado.
Regional molecular genetic key of thirteen snow pool Aedes species (Diptera:Culicidae) in northern Colorado.
复制标题
科罗拉多州北部 13 种雪池伊蚊（双翅目：库蚊科）的区域分子遗传关键。
DOI：
10.1093/jmedent/34.4.404
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发表时间：
1997
影响因子：
2.1
通讯作者：
Black4th,WC
中科院分区：
文献类型：
--
作者：
West,DF;Payette,T;Mundy,T;Black4th,WC
通过对4龄或雄性生殖器的显微镜检查，可以容易而准确地识别出雪池伊蚊。早期的幼虫和雌虫无法准确识别。我们证明，核核糖体DNA顺反子的内转录间隔区（ITS）中的限制性片段长度多态性（RFLP）对在北方科罗拉多发现的13种雪池伊蚊中的每一种都是特异性的。采用聚合酶链反应（PCR）扩增ITS区，用限制性内切酶MspI和EcoRI双酶切。13个种之间的消化片段的大小差异是如此之小，他们只能解决与聚丙烯酰胺凝胶电泳和可视化与银染色。利用PCR-RFLP技术建立了北方科罗拉多落基山脉物种检索表。其中三个物种收集在加州，并有相同的PCR-RFLP模式，表明在ITS的限制性位点可能是保守的一些物种。
Snow poolAedesmosquitoes are identified easily and accurately by microscopic examination of 4th instars or male genitalia. Early instars and females cannot be identified accurately. We demonstrate that restriction fragment-length polymorphisms (RFLP) in the internal transcribed spacer (ITS) of the nuclear ribosomal DNA cistron are specific to each of 13 snow poolAedesspecies found in northern Colorado. The ITS was amplified by the polymerase chain reaction (PCR) and digested withAluIandMspIrestriction endonucleases. Differences in the sizes of digested fragments among the 13 species were so slight that they could only be resolved with polyacrylamide gel electrophoresis and visualized with silver staining. A key to species found in the Rocky Mountains of northern Colorado was constructed using the PCR-RFLP patterns. Three of the species were collected in California and had identical PCR-RFLP patterns, indicating that restriction sites in the ITS may be conserved within some species.