Nitrous oxide (N2O) induces 2n gametes in sterile F1 hybrids between Oriental x Asiatic lily (Lilium) hybrids and leads to intergenomic recombination
Nitrous oxide (N2O) induces 2n gametes in sterile F1 hybrids between Oriental x Asiatic lily (Lilium) hybrids and leads to intergenomic recombination
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DOI：
10.1007/s10681-005-9032-0
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发表时间：
2006-04-01
期刊：
影响因子：
1.9
通讯作者：
Van Tuyl, Jaap M.
中科院分区：
文献类型：
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作者：
Barba-Gonzalez, Rodrigo;Miller, Chad T.;Van Tuyl, Jaap M.
以8个不同基因型的东方×亚洲百合（2n = 2x = 24）杂种F1代为材料，用N_2O气体加压处理24和48 h。在处理时，所有植物最古老花蕾的花粉囊都处于早期减数分裂阶段。通过花粉在体外萌发以及通过将它们用于与二倍体亚洲杂种（2n = 2x = 24）作为雄性和雌性亲本的杂交来监测来自处理植物的成熟花的育性。在五个八种基因型的OA杂种有证据表明，生产的2n花粉在体外发芽，从一个或两个治疗。三个基因型的2n花粉均成功地用于杂交。在两种情况下，经处理的植物成功地用作母本，这表明形成了2n（或2x）卵细胞。对N_2O处理的41个有性多倍体后代的分析表明，它们均为整倍体，其中三倍体34个（2n = 3x = 36），四倍体7个（2n = 4x = 48）。通过对12个后代植株的基因组原位杂交（GISH）进行详细的细胞学分析，证明N_2O在大多数情况下诱导了第一次分裂恢复配子，在两种情况下，它们通过不确定减数分裂恢复产生配子。有证据表明，基因组间重组在三个案件。
Eight different genotypes of the F-1 hybrids between Oriental x Asiatic lily (Lilium) hybrids (2n = 2x = 24) were treated with nitrous oxide (N2O) gas under pressure for 24 and 48 hours. At the time of treatment, all plants possessed early meiotic stages in the anthers of the oldest flower buds. The mature flowers from treated plants were monitored for fertility through pollen germination in vitro as well as by using them in crosses with diploid Asiatic hybrids (2n = 2x = 24) both as male and female parents. In five out of the eight genotypes of OA hybrids there was evidence for the production of 2n pollen which germinated in vitro from either one or both treatments. The 2n pollen from three genotypes was successfully used in crosses. In two cases, the treated plants were successfully used as female parents which indicated the formation of 2n (or 2x) egg cells. From an analysis of 41 sexual polyploid progenies obtained from N2O treated plants it was shown that they were all euploids consisting of 34 triploids (2n = 3x = 36) and seven tetraploids (2n = 4x = 48). A detailed cytological analysis of 12 progeny plants through genomic in situ hybridization (GISH) proved that N2O had induced first division restitution gametes in most cases and in two cases they produced gametes through indeterminate meiotic restitution. There was evidence for intergenomic recombination in three cases.