Taxol biosynthetic genes
Taxol biosynthetic genes
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DOI：
10.1016/s0031-9422(01)00160-1
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发表时间：
2001-09-01
期刊：
影响因子：
3.8
通讯作者：
Croteau, R
中科院分区：
文献类型：
--
作者：
Walker, K;Croteau, R
总结了从红豆杉 cDNA 文库中分离出来的、对紫杉醇生物合成具有特异性的异源表达全长 cDNA 克隆的功能和特性。重组酶被描述为催化该途径的早期步骤，包括紫杉二烯合酶、紫杉二烯-5α-醇-O-乙酰基转移酶和紫杉二烯-5α-乙酸酯IOP-羟化酶。以及催化后期步骤，包括 10-脱乙酰浆果赤霉素 III-10 β-O-乙酰基转移酶和紫杉烷 2 α-O-苯甲酰基转移酶。还描述了红豆杉香叶基香叶基二磷酸合酶的特性，尽管该合酶不介导紫杉醇生物合成的关键步骤，但它确实提供了通用质体二萜前体香叶基香叶基二磷酸，用于启动紫杉醇生物合成。 (C) 2001 Elsevier Science Ltd. 保留所有权利。
The function and properties of heterologously expressed full-length cDNA clones, isolated from a Taxus cDNA library and specific to Taxol biosynthesis, are summarized. Recombinant enzymes are described that catalyze early steps of the pathway, including taxadiene synthase, taxadin-5 alpha -ol-O-acetyltransferase and taxadien-5 alpha -yl acetate IOP-hydroxylase. and that catalyze late steps, including 10-deacetylbaccatin III-10 beta -O-acetyltransferase and taxane 2 alpha -O-benzoyltransferase. The properties of Taxus geranylgeranyl diphosphate synthase are also described, although this synthase does not mediate a committed step of Taxol biosynthesis, it does provide the universal plastidial diterpenoid precursor, geranylgeranyl diphosphate, for initiating Taxol biosynthesis. (C) 2001 Elsevier Science Ltd. All rights reserved.