Monitoring Chromatin Regulation in Planarians Using Chromatin Immunoprecipitation Followed by Sequencing (ChIP-seq).
Monitoring Chromatin Regulation in Planarians Using Chromatin Immunoprecipitation Followed by Sequencing (ChIP-seq).
复制标题
使用染色质免疫沉淀和测序 (ChIP-seq) 监测涡虫的染色质调控。
DOI：
10.1007/978-1-0716-2172-1_28
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Sridhar D
中科院分区：
文献类型：
--
作者：
Sridhar D
Planarians是研究动物再生和干细胞的一个可访问的模型系统。在过去的二十年里，新的分子技术为我们提供了强大的工具来了解全身再生和多能成体干细胞。我们描述了一种方法进行染色质免疫沉淀，然后测序（ChIP-seq）的Planarian细胞依赖于流式细胞仪分离不同的细胞群，然后免疫沉淀和下一代测序库的制备。组蛋白修饰的全基因组分析使得能够更好地理解发育、多能性和分化中的表观遗传机制。该方案增加了功能基因组方法的不断增长的列表，以研究动物的全身再生。
Planarians are an accessible model system to study animal regeneration and stem cells. Over the last two decades, new molecular techniques have provided us with powerful tools to understand whole-body regeneration and pluripotent adult stem cells specifically. We describe a method for performing Chromatin Immunoprecipitation followed by sequencing (ChIP-seq) on planarian cells that relies on FACS to isolate different cell populations followed by immunoprecipitation and library preparation for next-generation sequencing. Whole-genome profiling of histone modifications enables a greater understanding of epigenetic mechanisms in development, pluripotency, and differentiation. This protocol adds to the growing list of functional genomic approaches to study whole-body regeneration in animals.