The C. elegans vitellogenin genes: short sequence repeats in the promoter regions and homology to the vertebrate genes.
The C. elegans vitellogenin genes: short sequence repeats in the promoter regions and homology to the vertebrate genes.
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线虫卵黄原基因：启动子区域有短序列重复，与脊椎动物基因同源。
DOI：
10.1093/nar/13.14.5283
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发表时间：
1985
影响因子：
14.9
通讯作者：
Blumenthal,T
中科院分区：
文献类型：
--
作者：
Spieth,J;Denison,K;Kirtland,S;Cane,J;Blumenthal,T
小杆线虫含有一个卵黄蛋白原基因的小家族，该基因在雌雄同体成虫的肠道中大量表达。为了识别可能的调节元件，我们对六个基因中的五个基因的5 '末端周围的DNA进行了测序。包含在区域内已大大偏离彼此，两个不同的七聚体序列被发现重复内的第一个200 bp的每个基因的上游。第一个序列TGTCAAT在每个基因的上游至少有一次作为完美的七聚体存在。在每个5′侧翼区，它在两个方向上重复4到6次，允许一个碱基错配。第二个序列CTGATAA也作为一个完美的heptsmer存在于每个基因上游的限制性区域中。这两个序列元件可能参与了卵黄蛋白原基因的调控。值得注意的是，CTGATAA序列存在于脊椎动物卵黄蛋白原基因的启动子区中的类似位置。事实上，我们的数据揭示了线虫和脊椎动物卵黄原蛋白之间惊人的相似性。
The nematodeCaenorhabditiseleganscontains a small family of vitellogenin genes which is expressed abundantly, but only in the intestine of the adult hermaphrodite worm. In order to identify possible regulatory elements, we have sequenced the DNA surrounding the 5′ ends of five of the six genes. Contained within regions which have largely diverged from one another, two different heptameric sequences are found repeated within the first 200 bp upstream of each of the genes. The first sequence, TGTCAAT, is present as a perfect heptamer at least once upstream of each gene. It is repeated in both orientations four to six times in each 5′ flanking region, allowing a one-base mismatch. The second sequence, CTGATAA, is also present as a perfect heptsmer in a restricted region upstream of each gene. These two sequence elements may be involved in regulation of the vitellogenin genes. Remarkably, the CTGATAA sequence is present in a similar location in the promoter regions of vertebrate vitellogenin genes. In fact, our data reveal a surprising degree of similarity between the nematode and vertebrate vitellogenins.