Extraction, characterization of polysaccharides from lycium barbarum and its effect on bone gene expression in rats
Extraction, characterization of polysaccharides from lycium barbarum and its effect on bone gene expression in rats
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DOI：
10.1016/j.carbpol.2009.11.038
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发表时间：
2010-05-05
影响因子：
11.2
通讯作者：
Wang Caijiao
中科院分区：
文献类型：
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作者：
Zhu, Mengyong;He ChangSheng;Wang Caijiao
从枸杞干果中提取水溶性多糖。采用气相色谱-质谱联用仪（GC-MS）和傅立叶变换红外光谱（FT-IR）对枸杞多糖的化学成分进行了表征。GC-MS分析结果表明，枸杞多糖的单糖组成主要为葡萄糖和甘露糖。检测到微量木糖、半乳糖、阿拉伯糖和半乳糖。傅里叶变换红外光谱（FT-IR）显示了枸杞多糖的典型特征。药理研究表明枸杞多糖能提高大鼠骨密度和骨矿含量。RT-PCR研究表明LBP处理导致大鼠血液中Col 5a 2和Alpl的mRNA表达增加。蛋白质印迹分析引起LBP给药45天后Col 5a 2和Alpl蛋白水平降低。总之，我们的研究结果提供了证据，枸杞多糖增加骨基因表达，骨密度（BMD）和骨矿物质含量（BMC）在去卵巢大鼠。(C)2009爱思唯尔有限公司保留所有权利。
In this study, water-soluble polysaccharides were isolated from dried fruits of lycium barbarum. Gas chromatography-mass spectrophotometer (GC-MS) and Fourier-transform infrared (FT-IR) spectroscopy were used to characterize the compounds in polysaccharides from lycium barbarum (LBP). GC-MS analysis revealed that glucose and mannose were the main monosaccharides of LBP. Xylose, galactose, arabinose and galactose were detected in trace amounts. The Fourier-transform infrared spectra (FT-IR) of LBP revealed typical characteristics of polysaccharides. The pharmacological study indicated that LBP could enhance bone mineral density (BMD) and bone mineral content (BMC) in rats. RT-PCR studies had shown that LBP treatment resulted in increase in the mRNA expression of Col5a2 and Alpl in rat blood. Western blot analyses elicited a decreased Col5a2 and Alpl protein level after 45 days of LBP administration. Taken together, our results provide evidence that LBP increase bone gene expression, bone mineral density (BMD) and bone mineral content (BMC) in ovariectomized rats. (C) 2009 Elsevier Ltd. All rights reserved.