Investigation of six selected bacterial species in endo-periodontal lesions
Investigation of six selected bacterial species in endo-periodontal lesions
复制标题
DOI：
10.1111/j.1365-2591.2011.01974.x
复制
发表时间：
2012-03-01
影响因子：
5
通讯作者：
Stratul, S. I.
中科院分区：
文献类型：
--
作者：
Didilescu, A. C.;Rusu, D.;Stratul, S. I.
目的探讨微小微单胞菌、具核梭杆菌、直弯杆菌、结节真杆菌、腐蚀艾肯氏菌和生痰二氧化碳嗜纤维菌等6种橙子和绿色复合菌与牙周内膜病变的相关性。方法对46例不同类型的牙周内膜病变患者进行了调查。临床检查，根尖片和微生物采样的根管系统（endo）和牙周袋（perio）进行。通过聚合酶链反应（PCR）和DNADNA杂交（micro-IDent plus;海恩生命科学，德国）对细菌进行定性和半定量评价。结果根管标本中微小P.micra、具核F.nucleatum和生痰C.sputigena的细菌载量极高，而牙周标本中经常定植相同的菌种，加上直链C. rectus。核内梭菌和微小梭菌之间存在显著相关性（P = 0.03，Fisher精确检验）。存在以下之间的关联的边缘证据：（i）生痰梭菌-内多和生痰梭菌-外多（P = 0.06，Fisher精确检验）;（ii）微小内多和微小外多（P = 0.05，Fisher精确检验）。对叩诊的敏感性与微内疟原虫病例的机会增加相关（P = 0.03，Pearson卡方检验）。结论具核梭菌、微小丙酸杆菌和生痰梭菌可能参与了牙周内膜病变的发生。
Aim To investigate and determine possible associations of six tested bacteria belonging to orange and green complexes, in endo-periodontal lesions: Parvimonas micra, Fusobacterium nucleatum, Campylobacter rectus, Eubacterium nodatum, Eikenella corrodens and Capnocytophaga sputigena. Methodology Forty-six patients presenting with different types of endo-periodontal lesions were investigated. Clinical examinations, periapical radiographs and microbiological sampling from the canal system (endo) and periodontal pockets (perio) were performed. Qualitative and semiquantitative evaluation of bacteria was performed by polymerase chain reaction (PCR) and DNADNA hybridization (micro-IDent plus; Hain Lifescience, Germany). Results Extremely high bacterial loads in endodontic samples were recorded for P.micra, F.nucleatum and C.sputigena, while periodontal samples were often colonized by the same species, plus C.rectus. Significant association was recorded between F.nucleatum-endo and P.micra-endo (P = 0.03, Fishers exact test). There was marginal evidence of associations between: (i) C.sputigena-endo and C.sputigena-perio (P = 0.06, Fishers exact test); (ii) P.micra-endo and P.micra-perio (P = 0.05, Fishers exact test). Sensitivity to percussion was associated with an increased chance of cases with P.micra-endo (P = 0.03, Pearson chi-square test). Conclusion The findings suggest that F.nucleatum, P.micra and C.sputigena may play a role in the pathogenesis of endo-periodontal lesions.