Preparation of chains of magnetosomes, isolated from Magnetospirillum magneticum strain AMB-1 magnetotactic bacteria, yielding efficient treatment of tumors using magnetic hyperthermia
Preparation of chains of magnetosomes, isolated from Magnetospirillum magneticum strain AMB-1 magnetotactic bacteria, yielding efficient treatment of tumors using magnetic hyperthermia
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DOI：
10.1016/j.ijpharm.2012.06.015
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发表时间：
2012-09-15
影响因子：
5.8
通讯作者：
Chebbi, Imene
中科院分区：
文献类型：
--
作者：
Alphandery, Edouard;Guyot, Francois;Chebbi, Imene
从Magnetospirillum magneticum菌株AMB-1趋磁细菌中分离出磁小体链，在30 W下超声2 h检测磁热疗治疗肿瘤的效果。这些链是由磁小体组成的，磁小体通过由蛋白质构成的细丝相互连接。当它们与癌细胞一起孵育，并暴露在频率为198 kHz、平均磁场强度为20或30 mT的交变磁场中时，它们能有效抑制癌细胞的增殖。这种行为可以解释为高细胞内化，在溶液中的良好稳定性和磁小体链的均匀分布，从而实现有效的加热。当这些链在1% SDS的存在下在90℃下加热5小时时，将磁小体结合在一起的丝变性，得到单个的磁小体。与磁小体链相反，在交变磁场作用下，单个磁小体容易聚集，在溶液中不稳定，不能有效抑制癌细胞增殖。(C) 2012 Elsevier B.V.版权所有
Chains of magnetosomes isolated from Magnetospirillum magneticum strain AMB-1 magnetotactic bacteria by sonication at 30 W during 2 h are tested for magnetic hyperthermia treatment of tumors. These chains are composed of magnetosomes, which are bound to each other by a filament made of proteins. When they are incubated in the presence of cancer cells and exposed to an alternating magnetic field of frequency 198 kHz and average magnetic field strength of 20 or 30 mT, they produce efficient inhibition of cancer cell proliferation. This behavior is explained by a high cellular internalization, a good stability in solution and a homogenous distribution of the magnetosome chains, which enables efficient heating. When the chains are heated during 5 h at 90 degrees C in the presence of 1% SDS, the filament binding the magnetosomes together is denatured and individual magnetosomes are obtained. By contrast to the chains of magnetosomes, the individual magnetosomes are prone to aggregation, are not stable in solution and do not produce efficient inhibition of cancer cell proliferation under application of an alternating magnetic field. (C) 2012 Elsevier B.V. All rights reserved.