Protective effects of prebiotics inulin and lactulose from cytotoxicity and genotoxicity in human colon adenocarcinoma cells
Protective effects of prebiotics inulin and lactulose from cytotoxicity and genotoxicity in human colon adenocarcinoma cells
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DOI：
10.1016/j.foodres.2013.03.024
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发表时间：
2013-06-01
影响因子：
8.1
通讯作者：
Morgan, Winston A.
中科院分区：
文献类型：
--
作者：
Adebola, Oluwakemi;Corcoran, Olivia;Morgan, Winston A.
膳食中益生元的公认作用是改善益生菌在肠道中的存活和生长，但很少有研究调查益生元可能提供的好处超过其与益生菌的作用的可能性。本研究探讨益生素菊粉和乳果糖对人粪水、胆汁酸和4-硝基喹啉-1-氧化物(4-NQO)对人结肠癌细胞株HT-29的细胞毒性和遗传毒性的保护作用。用四甲基偶氮唑盐比色法测定，脱氧胆酸、石胆酸和50%粪水使细胞存活率分别下降75%、74%和50%。在菊粉和乳果糖(0.5-2.0%)存在下，细胞存活率在脱氧胆酸、石胆酸和粪水中分别增加100%、30%和40%。通过SOS显色试验发现，粪水(50%v/v)和4-NQO(1-2 g/ml)都具有高度的遗传毒性，但菊粉和乳果糖(2%)孵育后，粪水的SOS诱导效价(SOSIP)分别下降了70%和57%，4-NQO的SOSIP下降了29%和74%。用Ames波动试验观察到4-NQO对遗传毒性的类似保护作用。这些测试表明，在没有益生菌的情况下，益生元能够保护哺乳动物细胞(HT-29细胞)免受细胞毒性，并在细菌突变检测中降低已知诱变剂的遗传毒性。在体内复制这些结果将表明，在饮食中加入益生元可能直接带来与益生菌生存和生长相关的健康益处。(C)2013爱思唯尔有限公司。保留所有权利。
The accepted role of dietary prebiotics is to improve probiotic survival and growth in the lower intestine, yet few studies have investigated the possibility that prebiotics may offer benefits beyond their role with probiotics. This study investigates the protective effects of the prebiotics inulin and lactulose against cytotoxic and genotoxic effects in HT-29 human adenocarcinoma colon cells caused by human faecal water, bile acids and 4-nitroquinoline-1-oxide (4-NQO). Using the MTT assay, deoxycholic acid, lithocholic acid and faecal water 50% v/v reduced cell viability by 75,74 and 50% respectively. In the presence of inulin and lactulose (0.5-2.0%) cell survival increased by 100% for deoxycholic acid, by 30% for lithocholic acid and by 40% for faecal water. Both faecal water (50% v/v) and 4-NQO (1-2 g/ml) were found to be highly genotoxic using the SOS chromotest, however incubation with inulin and lactulose (2%) significantly reduced the bacterial assay SOS inducing potency (SOSIP) by 70% and 57% respectively for faecal water and by up to 29 and 74% for 4-NQO. Similar protection against genotoxicity was observed with 4-NQO using the Ames fluctuation test. These tests show that prebiotics, in the absence of probiotics, were able to protect mammalian cells (HT-29 cells) against cytotoxicity and reduce the genotoxicity of known mutagens in bacterial mutation assays. Reproduction of these results in vivo would suggest that the inclusion of prebiotics in diet may directly confer health benefits beyond those associated with probiotic survival and growth. (C) 2013 Elsevier Ltd. All rights reserved.