Immunolocalization of tissue non-specific alkaline phosphatase in mice
Immunolocalization of tissue non-specific alkaline phosphatase in mice
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DOI：
10.1007/s004180050103
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发表时间：
1997-03-01
影响因子：
2.3
通讯作者：
Ozawa, H
中科院分区：
文献类型：
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作者：
Hoshi, K;Amizuka, N;Ozawa, H
在小鼠组织中检测了组织非特异性碱性磷酸酶（TNAP）的免疫定位，采用TNAP的特异性抗血清对冷冻切片、50 μ m组织切片和石蜡切片进行检测。TNAP在包括骨、软骨和牙齿的硬组织中以高水平检测。在骨组织中，TNAP免疫反应位于前成骨细胞的整个细胞表面，以及成骨细胞的基底外侧细胞膜。它也定位于一些静止的软骨细胞和大多数增殖和肥大的软骨细胞。在门牙中，中间层、成牙本质细胞下层、分泌性成釉细胞近端部分和成牙本质细胞基底外侧部分的细胞表现出特别强的免疫反应性。在其他软组织中观察到免疫反应性，如肾脏近端肾小管刷状缘，肝脏胆小管细胞膜和胎盘滋养细胞。这些免疫定位是非常相似的酶组织化学定位。然而，无论是下颌下腺和肠，这两个碱性磷酸酶活性的酶组织化学，揭示TNAP的免疫反应性。因此，TNAP的免疫组织化学研究使我们能够定位TNAP同工酶，从而区分它从其他同工酶。
Immunolocalization of tissue non-specific alkaline phosphatase (TNAP) was examined in murine tissues, employing a specific antiserum to TNAP on frozen sections, 50-mu m tissue slices: and paraffin sections. TNAP was detected at high levels in hard tissues including bone, cartilage, and tooth. In bone tissue, the TNAP immunoreactivity was localized on the entire cell surface of preosteoblasts, as well as the basolateral cell membrane of osteoblasts. It was also localized on some resting chondrocytes and most of the proliferative and hypertrophic cells in cartilage. In the incisor, cells of the stratum intermedium, the subodontoblastic layer, the proximal portion of secretory ameloblasts, and the basolateral portion of odontoblasts showed particularly strong immunoreactivity. Immunoreactivity was observed in other soft tissues, such as the brush borders of proximal renal tubules in kidney, on cell membrane of the biliary canalicula in liver and in trophoblasts in the placenta. These immunolocalizations were quite similar to enzyme histochemical localizations. However, neither the submandibular gland nor the intestine, which both exhibited alkaline phosphatase activity by enzyme histochemistry, revealed immunoreactivity for TNAP Therefore, immunocytohistochemical studies for TNAP enabled us to localize the TNAP isozyme, thus distinguishing it from other isozymes.