Susceptibility to flavivirus-specific antiviral response of Oas1b affects the neurovirulence of the Far-Eastern subtype of tick-borne encephalitis virus
Susceptibility to flavivirus-specific antiviral response of Oas1b affects the neurovirulence of the Far-Eastern subtype of tick-borne encephalitis virus
复制标题
Oas1b 对黄病毒特异性抗病毒反应的敏感性影响蜱传脑炎病毒远东亚型的神经毒力
DOI：
10.1007/s00705-012-1579-1
复制
发表时间：
2013
影响因子：
2.7
通讯作者：
Ikuo Takashima
中科院分区：
文献类型：
--
作者：
Kentaro Yoshii;Kanako Moritoh;Noriyo Nagata;Kana Yokozawa;Mizuki Sakai;Nobuya Sasaki;Hiroaki Kariwa;Takashi Agui;Ikuo Takashima
蜱传脑炎病毒（TBEV）是一种人畜共患病原体，可导致人类致命性脑炎。2 '-5'-寡腺苷酸合成酶1b（Oas 1b）是黄病毒的一个抗性基因，但大多数近交系小鼠不具有功能性的Oas 1b基因。本研究利用一株携带Oas 1b功能基因的同源株B6. MSM-Oas对远东TBEV的致病性进行了研究。虽然Oshima 5-10脑内感染B6. MSM-Oas小鼠导致有限的疾病体征，但Sofjin-HO感染导致死亡和严重的神经学体征。当Oshima 5-10从大脑中清除时，尽管完整Oas 1b基因的表达水平相似，但Sofjin-HO没有被清除。在感染Sofjin-HO的脑中观察到具有病毒抗原的坏死神经元和炎症反应。这些数据表明，不同的敏感性Oas 1b的抗病毒活性导致了不同的神经毒力在两个TBEV株。
Tick-borne encephalitis virus (TBEV) is a zoonotic agent that causes fatal encephalitis in humans. 2’-5’-oligoadenylate synthetase 1b (Oas1b) has been identified as a flavivirus resistance gene, but most inbred laboratory mice do not possess a functionalOas1bgene. In this study, a congenic strain carrying a functionalOas1bgene, B6.MSM-Oas, was used to evaluate the pathogenicity of Far-Eastern TBEV. Although intracerebral infection of B6.MSM-Oasmice by Oshima 5-10 resulted in limited signs of illness, infection by Sofjin-HO resulted in death with severe neurologic signs. While Oshima 5-10 was cleared from the brain, Sofjin-HO was not cleared despite a similar level of expression of the intactOas1bgene. Necrotic neurons with viral antigens and inflammatory reactions were observed in the brain infected with Sofjin-HO. These data indicate that the different susceptibility to the antiviral activity of Oas1b resulted in a difference in neurovirulence in the two TBEV strains.