Local complement activation in aqueous humor in patients with age-related macular degeneration
Local complement activation in aqueous humor in patients with age-related macular degeneration
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DOI：
10.1038/eye.2016.328
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发表时间：
2017-05-01
期刊：
影响因子：
3.9
通讯作者：
Fauser, S.
中科院分区：
文献类型：
--
作者：
Schick, T.;Steinhauer, M.;Fauser, S.
目的 研究新生血管性年龄相关性黄斑变性 (nAMD) 患者房水和血浆中的补体激活情况。 患者和方法 收集 31 名 nAMD 患者和 30 名年龄匹配对照者的房水和 EDTA 血浆。测量补体因子 3 (C3)、调节因子 H (FH) 和因子 I (FI) 以及补体激活产物 Ba、C3a 和末端补体复合物 (sC5b-9) 的水平。使用Mann-Whitney U-test确定补体水平和表型之间的关联。结果在血浆中，nAMD组和对照组之间没有发现显着差异。在房水中，Ba (P = 0.002) 和 C3a (P = 0.002) 水平显着升高表明 nAMD 患者局部补体激活，以及补体调节剂 FH (P = 0.02) 和 FI (P = 0.04) 伴随上调的趋势。 结论 我们的研究结果为 nAMD 患者局部补体失调提供了强有力的证据。
Purpose To investigate complement activation in aqueous humor and in plasma of patients with neovascular age-related macular degeneration (nAMD).Patients and methods Aqueous humor and EDTA-plasma of 31 nAMD patients and 30 age-matched controls was collected. The levels of the complement factor 3 (C3), the regulators factor H (FH), and factor I (FI), and of the complement activation products Ba, C3a, and the terminal complement complex (sC5b-9) were measured. Associations between complement levels and phenotype were determined using Mann-Whitney U-test.Results In plasma, no significant differences were found between the nAMD group and the control group. In aqueous humor, significantly increased levels of Ba (P= 0.002), and C3a (P= 0.002) indicate local complement activation in nAMD patients and a trend for a concomitant upregulation of the complement regulators FH (P= 0.02) and FI (P= 0.04).Conclusions Our findings provide strong evidence for a local complement dysregulation in nAMD patients.