Identification of Plant Extracts that Inhibit the Formation of Diabetes-Linked IAPP Amyloid.
Identification of Plant Extracts that Inhibit the Formation of Diabetes-Linked IAPP Amyloid.
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DOI：
10.1016/j.hermed.2015.11.001
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发表时间：
2016-03
影响因子：
2.3
通讯作者：
Moffet DA
中科院分区：
文献类型：
--
作者：
Fuentes AL;Hennessy K;Pascual J;Pepe N;Wang I;Santiago A;Chaggan C;Martinez J;Rivera E;Cota P;Cunha C;Nogaj LA;Moffet DA
筛选了27种蔬菜、香料和草药的提取物，以确定其抑制胰岛淀粉样多肽（IAPP，amylin）聚集成毒性淀粉样蛋白聚集体的功能能力。IAPP的聚集与2型糖尿病中胰岛β细胞的死亡直接相关。抑制IAPP的聚集被认为有可能减缓（如果不能完全预防）这种疾病的进展。由于已知蔬菜、香料和草药具有许多不同的积极健康作用，因此筛选了27种植物（在美国丰富并跨越几个植物科）的提取物抑制有毒IAPP聚集体形成的能力。通过（1）硫磺素T结合试验，（2）使用原子力显微镜观察淀粉样蛋白纤维和（3）细胞拯救研究评估其抗淀粉样蛋白活性。从这项研究中，薄荷，薄荷，红甜椒和百里香被认为具有最大的抗淀粉样蛋白活性。
The extracts of 27 vegetables, spices and herbs were screened for their functional ability to inhibit the aggregation of islet amyloid polypeptide (IAPP, amylin) into toxic amyloid aggregates. The aggregation of IAPP has been directly linked to the death of pancreatic β-islet cells in type 2 diabetes. Inhibiting the aggregation of IAPP is believed to have the potential to slow, if not prevent entirely, the progression of this disease. As vegetables, spices and herbs are known to possess many different positive health effects, the extracts of 27 plants (abundant within the United States and spanning several plant families) were screened for their ability to inhibit the formation of toxic IAPP aggregates. Their anti-amyloid activities were assessed through (1) thioflavin T binding assays, (2) visualization of amyloid fibers using atomic force microscopy and (3) cell rescue studies. From this research, mint, peppermint, red bell pepper and thyme emerged as possessing the greatest anti-amyloid activity.