Parahydrogen-Induced 13C Hyperpolarizer Using a Flow Guide for Magnetic Field Cycling to Evoke 1H-13C Spin Order Transfer Toward Metabolic MRI
Parahydrogen-Induced 13C Hyperpolarizer Using a Flow Guide for Magnetic Field Cycling to Evoke 1H-13C Spin Order Transfer Toward Metabolic MRI
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DOI：
10.1109/tbme.2024.3365195
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发表时间：
2024-07-01
影响因子：
4.6
通讯作者：
Matsumoto，Shingo
中科院分区：
文献类型：
--
作者：
Sawami，Koudai;Naganuma，Tatsuya;Matsumoto，Shingo
目的分子氢的单重态--对氢原子与不饱和前体分子通过对氢诱导极化(PhIP)引起两个1H原子核自旋的超极化。随后通过磁场循环(MFC)从1H到周围13C核自旋的自旋顺序转移(SOT)导致了13C磁共振成像(MRI)中信号的显著增强。在这里，我们报道了一种独特的PHIP13C超极化器系统的发展，使用MFC的流动导向器。方法利用13C标记的代谢示踪剂的J偶联值，对1H到13C自旋顺序转移的最佳MFC方案进行了量子化学模拟。基于模拟的MFC方案和零场室中预先测量的磁场分布，对导流系统进行了三维设计。结果当对氢示踪剂以指定的流量通过导流系统时，该系统有效地将超极化的1H的自旋顺序转移到特定的13C自旋。在13C标记的丙酮酸和富马酸中，13C磁共振信号增强了4万多倍，与1.5T时的热平衡水平相比，可以用于进行小鼠的活体代谢磁共振成像。结论研制了一种基于PHIP的全自动13C偏振器，使用独特的流程指南进行1H-13C SOT的MFC。
ObjectiveThe pair-wise addition of parahydrogen, the singlet form of molecular hydrogen, to unsaturated precursors evokes the hyperpolarization of two parahydrogen-derived1H nuclear spins through a process known as parahydrogen-induced polarization (PHIP). Subsequent spin order transfer (SOT) from the1H to the surrounding13C nuclear spins via magnetic field cycling (MFC) results in substantial signal enhancement in13C magnetic resonance imaging (MRI). Here, we report the development of a unique PHIP13C hyperpolarizer system using a flow guide for MFC.MethodsThe optimal MFC scheme for1H to13C spin order transfer was quantum-chemically simulated using the J-coupling values of13C-labeled metabolic tracers. The flow guide system was three-dimensionally designed based on the simulated MFC scheme and pre-measured magnetic field distribution in a zero-field chamber.ResultsThe system efficiently transfers the spin order of hyperpolarized1H to a particular13C spin when the parahydrogenated tracer passes through the flow guide at a designated flow rate. The13C MRI signal is enhanced more than 40,000 times in13C-labeled pyruvate and fumarate, compared to the thermal equilibrium level at 1.5 T, was achieved for conductingin vivometabolic MRI of mice.ConclusionA fully automated PHIP-based13C polarizer was developed using a unique flow guide to conduct the MFC for1H to13C SOT.SignificanceThe PHIP hyperpolarizer with a flow guide can conduct efficient1H-13C SOT without a MFC magnetic field sweep system and offers a cost-effective alternative to conventional dynamic nuclear polarization.