Preparation and Characterization of Chitosan/β-Glycerophosphate Thermal-Sensitive Hydrogel Reinforced by Graphene Oxide
Preparation and Characterization of Chitosan/β-Glycerophosphate Thermal-Sensitive Hydrogel Reinforced by Graphene Oxide
复制标题
氧化石墨烯增强壳聚糖/β-磷酸甘油热敏水凝胶的制备及表征
DOI：
10.3389/fchem.2018.00565
复制
发表时间：
2018-11-22
影响因子：
5.5
通讯作者：
Pei, Xibo
中科院分区：
文献类型：
--
作者：
Qin, Han;Wang, Jian;Pei, Xibo
基于壳聚糖（CS）和β-甘油磷酸盐（GP）的热敏水凝胶显示出良好的生物相容性和生物降解性。但由于其力学性能较差，限制了其应用。近年来，氧化石墨烯（GO）被广泛用作增强剂，与不同的聚合物制备纳米复合材料，以改善材料的性能。在本研究中，制备了具有不同GO/CS重量比（0.5%、1%、2%）的CS/GP基水凝胶。通过试管倒置法评价水凝胶在体温下的凝胶化时间。通过流变学测量监测加热过程中的凝胶化过程。采用扫描电子显微镜、液体置换法、傅立叶变换红外光谱和重量法对冻干水凝胶的形貌、孔隙率、化学结构和溶胀性能进行了研究。通过流变学测试和无侧限压缩试验分析了水凝胶的力学性能。使用MC 3 T3-E1小鼠前成骨细胞系来评估水凝胶的生物学特性。从这些评估中获得的结果表明，将GO添加到CS/GP中改善了所制备的水凝胶的性质，而不改变水凝胶的高多孔和互连的微观结构和溶胀能力。加入少量GO（CS重量比为0.5%）后，在体温下的凝胶化时间显著缩短近20%。含GO的水凝胶的力学性能比CS/GP的水凝胶的力学性能显著提高。在凝胶化温度下，GO水凝胶的储能模量（G ′）/损耗模量（G ″）是CS/GP的1.12 ~ 1.69倍。0.5%GO/CS/GP水凝胶的杨氏模量是CS/GP的1.76倍。此外，0.5%GO/CS/GP水凝胶显示出显著的生物亲和性，如细胞附着、存活力和增殖。这些结果表明，0.5%GO/CS/GP水凝胶在生物医学领域具有很大的实际应用潜力。
Thermal-sensitive hydrogel based on chitosan (CS) and beta-glycerophosphate (GP) has shown good biocompatibility and biodegradability. But the application of such hydrogel is limited due to its poor mechanical property. Recently, graphene oxide(GO) is widely used as a reinforcement agent to prepare nanocomposites with different polymers for improving the properties of the materials. In this study, CS/GP-based hydrogels with different weight ratio of GO/CS (0.5, 1, 2%) were fabricated. The gelation time of the hydrogels at body temperature was evaluated by tube inverting method. The gelation process during heating was monitored by rheological measurement. The morphology, porosities, chemical structure, swelling properties of the lyophilized hydrogels were investigated by scanning electron microscopy, liquid displacement method, Fourier transform infrared spectroscopy and gravimetric method. Mechanical property of the hydrogels was analyzed by rheological measurement and unconfined compression test. MC3T3-E1 mouse pre-osteoblast cell line was used to assess the biological properties of the hydrogels. The results obtained from those assessments revealed that the addition of GO into CS/GP improved the properties of the prepared hydrogels without changing the high porous and interconnected microstructure and swelling ability of the hydrogels. The gelation time at body temperature was significantly reduced by nearly 20% with the addition of small amount of GO (0.5% weight ratio of CS). The mechanical properties of the hydrogels containing GO were improved significantly over that of CS/GP. The storage (G')/loss (G '') moduli of the hydrogels with GO were 1.12 to 1.69 times that of CS/GP at the gelling temperature. The Young's modulus of 0.5%GO/CS/GP hydrogel is 1.76 times that of CS/GP. Moreover, the 0.5%GO/CS/GP hydrogel revealed remarkable biological affinity such as cellular attachment, viability and proliferation. All of these results suggest that 0.5%GO/CS/GP hydrogel has great potential for practical application in biomedical field.