Cementum protein 1 (CEMP1) induces a cementoblastic phenotype and reduces osteoblastic differentiation in periodontal ligament cells
Cementum protein 1 (CEMP1) induces a cementoblastic phenotype and reduces osteoblastic differentiation in periodontal ligament cells
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DOI：
10.1002/jcp.22770
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发表时间：
2012-02-01
影响因子：
5.6
通讯作者：
Morita, Ikuo
中科院分区：
文献类型：
--
作者：
Komaki, Motohiro;Iwasaki, Kengo;Morita, Ikuo
牙骨质是覆盖在牙根表面的钙化组织，具有刚性的固牙结构。牙周韧带是一种独特的非矿化结缔组织，是骨水泥母细胞和成骨细胞等矿化组织形成细胞的来源。CEMP1是一种新的骨质成分，其存在似乎仅限于成骨水泥细胞及其祖细胞。为了了解CEMP1的功能，我们研究了CEMP1在人牙周韧带细胞分化过程中的表达。免疫磁富集碱性磷酸酶（ALP）阳性牙周韧带细胞优先表达CEMP1。牙周膜细胞向成骨细胞分化时，CEMP1表达减少。CEMP1在牙周韧带细胞中的过度表达增强了成骨水泥细胞的分化，减弱了牙周细胞和成骨细胞的表型。我们的数据首次证明CEMP1不仅是成水泥细胞相关细胞的标记蛋白，而且还调节牙周韧带细胞的成水泥细胞功能。j .细胞。中国生物医学工程学报，2016,33(2):557 - 557。(C) 2011 Wiley期刊公司
Cementum is a calcified tissue covering the tooth root surface, which functions as rigid tooth-anchoring structure. Periodontal ligament is a unique non-mineralized connective tissue, and is a source of mineralized tissue forming cells such as cementoblasts and osteoblasts. The CEMP1 is a novel cementum component the presence of which appears to be limited to cementoblasts and their progenitors. In order to understand the function of CEMP1, we investigated CEMP1 expression during the differentiation of human periodontal ligament cells. Immunomagnetically enriched alkaline phosphatase (ALP)-positive periodontal ligament cells preferentially expressed CEMP1. CEMP1 expression was reduced when periodontal ligament cells differentiated to osteoblasts in vitro. Over-expression of CEMP1 in periodontal ligament cells enhanced cementoblast differentiation and attenuated periodontal and osteoblastic phenotypes. Our data demonstrate for the first time that the CEMP1 is not only a marker protein for cementoblast-related cells, but it also regulates cementoblast commitment in periodontal ligament cells. J. Cell. Physiol. 227: 649657, 2012. (C) 2011 Wiley Periodicals, Inc.