Development of a novel radiation imaging detector system for in vivo gene imaging in small animal studies
Development of a novel radiation imaging detector system for in vivo gene imaging in small animal studies
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DOI：
10.1109/23.685298
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发表时间：
1998-06-01
影响因子：
1.8
通讯作者：
Saha, MS
中科院分区：
文献类型：
--
作者：
Weisenberger, AG;Bradley, EL;Saha, MS
我们报告了辐射成像技术原型的初步结果，该技术利用放射性同位素碘 125 (I-125) 的发射特性作为探针，探测器系统采用晶体闪烁体和位置敏感光电倍增管。碘 125 通过电子捕获衰变，发射 35 keV 伽马射线，并立即发射数条 27-32 keV K α 和 K β 壳层 X 射线。因此，可以设置重合条件来检测 I-125 衰变，从而减少背景辐射对图像的贡献。我们报告的原型探测器由于闪烁体和光电倍增管的尺寸而具有有限的灵敏度和检测区域，设置它表现得足够好，足以证明这种方法在小鼠中成像 I-125 的可行性，甲状腺和褪黑激素结合的碘摄取的小鼠成像研究已经通过该探测器系统使用单独剂量的 I-125 或附着在褪黑激素上完成，分子生物学中的许多研究都遵循基因在不同阶段的表达和调节。生物体的发育或在不同的生理条件下。通过利用 I-125 标记的基因探针，分子生物学研究可以从该检测系统中受益。
We report preliminary results from a prototype of radiation imaging technology which takes advantage of the emission properties of the radioisotope iodine 125 (I-125) as the probe, The detector system utilizes crystal scintillators and a position sensitive photomultiplier tube. Iodine 125 decays via electron capture emitting a 35-keV gamma ray with the prompt emission of several 27-32-keV K alpha and K beta shell X rays. Because of this, a coincidence condition can be set to detect the I-125 decay, thus reducing background radiation contribution to the image. The prototype detector we report has a limited sensitivity and detection area because of the size of the scintillators and photomultiplier tubes, Set it performed well enough to demonstrate the viability of this method for imaging I-125 in a mouse, Mouse imaging studies of iodine uptake by the thyroid and melatonin binding have been done with this detector system using doses of I-125 alone or attached to the melatonin, Many studies in molecular biology follow the expression and regulation of a gene at different stages of an organism's development or under different physiological conditions. Molecular biology research could benefit from this detection system by utilizing I-125-labeled gene probes.