Immunization with a peptide corresponding to chlamydial heat shock protein 60 increases the humoral immune response in C3H mice to a peptide representing variable domain 4 of the major outer membrane protein of Chlamydia trachomatis.
Immunization with a peptide corresponding to chlamydial heat shock protein 60 increases the humoral immune response in C3H mice to a peptide representing variable domain 4 of the major outer membrane protein of Chlamydia trachomatis.
复制标题
用对应于衣原体热休克蛋白60的肽进行免疫增强了C3H小鼠中对代表沙眼衣原体主要外膜蛋白可变结构域4的肽的体液免疫应答。
DOI：
10.1128/cdli.6.3.356-363.1999
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Peterson,EM
中科院分区：
文献类型：
--
作者：
Motin,VL;delaMaza,LM;Peterson,EM
C3H （H-2k）小鼠易受人类沙眼衣原体菌株阴道攻击，因此是测试潜在衣原体疫苗候选菌株的有用菌株。然而，C3H小鼠对主要外膜蛋白（MOMP）可变结构域（VDs）的潜在保护肽免疫产生的抗体水平反应相当差。另一方面，C57BL/6 （H-2b）小鼠对阴道攻击具有中等抵抗力，但对衣原体MOMP VDs反应良好。代表通用t细胞辅助表位的多肽被用来确定在C3H和C57小鼠中是否可以增强对代表MOMP的VD4的肽的抗体反应，该肽已被证明含有中和表位。来自破伤风毒素的通用t细胞辅助肽、乙型肝炎病毒的前s2区、小鼠热休克蛋白60以及相应的衣原体热休克蛋白60 （hspct）片段与VD4肽共给药。多肽被包被在含有佐剂单磷酰脂A的脂质体中，并通过粘膜和肌肉注射的组合给药。通过抗体水平、体外中和试验和t细胞增殖来判断，唯一改善免疫应答的t细胞辅助肽是hspct。单独使用VD4时，C57BL/6菌株的hsptover水平没有显著提高；然而，C3H小鼠血清抗c抗体水平。沙眼增加到C57小鼠。然而，分子特异性和免疫球蛋白亚类分布与C57反应不同，中和滴度和t细胞增殖反应较低。在两株小鼠中，阴道抗体c滴度。trachomatiswere低。综上所述，在使用的t辅助肽中，只有hspct显著增强了C3H小鼠对VD4肽的免疫应答，而对C57小鼠的免疫应答只有适度的影响。
C3H (H-2k) mice are susceptible to a vaginal challenge with human strains ofChlamydia trachomatisand thus are a useful strain for testing potentialChlamydiavaccine candidates. However, C3H mice are fairly poor responders in terms of the level of antibody resulting from immunization with potential protective peptides representing variable domains (VDs) of the major outer membrane protein (MOMP). C57BL/6 (H-2b) mice, on the other hand, are moderately resistant to a vaginal challenge but are good responders to the chlamydial MOMP VDs. Peptides representing universal T-cell helper epitopes were employed to determine whether the antibody response to a peptide representing VD4 of the MOMP, which has been shown to contain neutralizing epitopes, could be enhanced in C3H and C57 mice. Universal T-cell helper peptides from tetanus toxin, the pre-S2 region of hepatitis B virus, and the mouse heat shock protein 60, as well as the corresponding segment of theChlamydiaheat shock protein 60 (hspct), were coadministered with the VD4 peptide. Peptides were coencapsulated in liposomes containing the adjuvant monophosphoryl lipid A and administered by using a combination of mucosal and intramuscular injection. The only T-cell helper peptide that improved the immune response as judged by antibody level, in vitro neutralization assays, and T-cell proliferation was hspct. The response in the C57BL/6 strain was not significantly enhanced with hspctover levels achieved with VD4 alone; however, in C3H mice the levels of serum antibody toC. trachomatisincreased to that seen in C57 mice. However, the molecular specificity and immunoglobulin subclass distribution differed from those of the C57 response, and the neutralizing titers and T-cell proliferation responses were lower. In both strains of mice, titers of vaginal antibody toC. trachomatiswere low. In summary, of the T-helper peptides used, only hspctsignificantly enhanced the immune response of C3H mice to the VD4 peptide, but it had only a modest effect on the immune response of C57 mice.