Biocompatible Shaped Particles from Dried Multilayer Polymer Capsules
Biocompatible Shaped Particles from Dried Multilayer Polymer Capsules
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DOI：
10.1021/bm4008666
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发表时间：
2013-11-01
影响因子：
6.2
通讯作者：
Kharlampieva, Eugenia
中科院分区：
文献类型：
--
作者：
Chen, Jun;Kozlovskaya, Veronika;Kharlampieva, Eugenia
我们展示了一种简单易用的方法来制备生物相容的单分散可控几何形状的中空微粒。通过干燥氢键聚N-乙烯基吡咯烷酮/单宁酸(PVPON/TA)(N)多层胶囊，得到了半球形、球形和立方体的微球。干燥球形胶囊可得到半球形颗粒。这种形状变化由胶囊硬度控制，由层数、胶囊直径和PVPON相对分子质量调节。当n>；=25.5时，可以得到在干态下保持其三维形状的立方和球形空心粒子。从完全坍塌的(PVPON/TA)(5.5)到干燥的自支撑(PVPON/TA)(25.5)尺寸为2微米的颗粒需要增加17倍的硬度。所有的中空颗粒可以进一步悬浮在水溶液中，同时在重新水化时保持其形状。利用人类癌细胞进行的细胞生长和活性研究表明，(PVPON/TA)多层粒子具有非细胞毒性特性。球形和半球形胶囊都被巨噬细胞内化，每个细胞摄取半球形颗粒的效率是巨噬细胞的两倍。本文提出的方法允许稳健地制备生物相容的形状颗粒，其形状和尺寸可以通过控制胶囊大小和壁厚轻松地调节。所报道的结构可用于生物医学应用，如形状控制的细胞摄取和流动动力学。
We demonstrated a simple and facile approach to fabricate biocompatible monodisperse hollow microparticles of controlled geometry. The hemispherical, spherical, and cubical microparticles are obtained by drying multilayer capsules of hydrogen-bonded poly(N-vinylpyrrolidone)/tannic acid (PVPON/TA)(n). Drying spherical capsules results in hemispherical particles if 15 < n < 20. This shape transformation is controlled by capsule stiffness, which is regulated by the layer number, capsule diameter, and PVPON molecular weight. Cubical and spherical hollow particles maintaining their three-dimensional shapes in the dry state are obtained if n >= 25.5. A 17-fold stiffness increase is required to lead from totally collapsed (PVPON/TA)(5.5) to dried self-supporting (PVPON/TA)(25.5) particles of 2 mu m in dimensions. All hollow particles could be further resuspended in aqueous solutions while retaining their shapes upon rehydration. The cell growth and viability studies using human cancer cells revealed noncytotoxic properties of the (PVPON/TA) multilayer particles. Both spherical and hemispherical capsules were internalized by macrophages with the uptake of the hemispherical particles per cell two times more efficient. The method presented here allows for a robust preparation of biocompatible shaped particles whose shape and dimensions can be easily tuned by controlling capsule size and wall thickness. The reported structures can be potentially useful for biomedical applications such as shape-controlled cellular uptake and flow dynamics.