T cell activation in abnormal perinatal events
T cell activation in abnormal perinatal events
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DOI：
10.1111/j.1348-0421.2009.00181.x
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发表时间：
2010-01-01
影响因子：
2.6
通讯作者：
Uchiyama, Takehiko
中科院分区：
文献类型：
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作者：
Matsuda, Yoshio;Kato, Hidehito;Uchiyama, Takehiko
本研究的目的是确定CD 45 RO + T细胞在脐带血中的百分比从出生在小于37周的妊娠新生儿。59例患者入组本研究，包括49例早产和10例足月分娩。早产分为两类：自发性（A组，n = 31）和指征性（B组，n = 18）。围产期感染分为C-CAM、H-CAM和新生儿感染。使用流式细胞术评估脐带中CD 45 RO + T细胞的百分比。使用ELISA测量IL-6。A组中，围产期感染组（n = 18）的CD 45 RO + T细胞百分率和IL-6浓度均显著高于无围产期感染组（n = 13）。脐血CD 45 RO + T细胞百分率与IL-6浓度呈显著相关（r = 0.62，P = 0.001）。B组7例羊水呈粉红色。在这些情况下，注意到CD 45 RO + T细胞的百分比增加（> 10%）。在无围产期感染的足月分娩孕妇中（n = 32），CD 45 RO + T细胞百分率与分娩时孕周无相关性（r =-0.139，P = 0.448）。我们的结论是，CD 45 RO+脐带血T细胞的高比例不仅在围产期感染，但也在围产期异常事件，如母亲出血在早产的存在。
The aim of this study was to determine the percentage of CD45RO+ T cells in umbilical cord blood from neonates born at less than 37 weeks of gestation. Fifty-nine patients were enrolled in this study, including 49 with preterm and 10 with term deliveries. Preterm deliveries were divided into two categories; spontaneous (Group A, n = 31) and indicated (Group B, n = 18). Perinatal infection was categorized as C-CAM, H-CAM and neonatal infection. The percentage of CD45RO+ T cells in the umbilical cord was assessed using flow cytometry. IL-6 was measured using ELISA. In Group A, the percentage of CD45RO+ T cells and concentrations of IL-6 in patients with perinatal infection (n = 18) were significantly higher than in those without perinatal infection (n = 13). A significant correlation between percentage of CD45RO+ T cells and IL-6 concentrations was observed in the cord blood (r = 0.62, P = 0.001). In Group B, pink-tinged amniotic fluid was observed in seven cases. In these cases, an increase in the percentage of CD45RO+ T cells (> 10%) was noted. In the cases without perinatal infection, which included all those delivered at term (n = 32), no correlation was observed between the percentage of CD45RO+ T cells and gestational age at delivery (r = -0.139, P = 0.448). We concluded that a high percentage of CD45RO+ cord blood T cells is observed not only in perinatal infection, but also in the presence of abnormal perinatal events such as maternal bleeding in preterm gestation.