UNIQUE COMPOSITION OF PLASTID CHAPERONIN-60 - ALPHA-POLYPEPTIDE-ENCODING AND BETA-POLYPEPTIDE-ENCODING GENES ARE HIGHLY DIVERGENT
UNIQUE COMPOSITION OF PLASTID CHAPERONIN-60 - ALPHA-POLYPEPTIDE-ENCODING AND BETA-POLYPEPTIDE-ENCODING GENES ARE HIGHLY DIVERGENT
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DOI：
10.1016/0378-1119(90)90385-5
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发表时间：
1990-10-15
期刊：
影响因子：
3.5
通讯作者：
HEMMINGSEN, SM
中科院分区：
文献类型：
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作者：
MARTEL, R;CLONEY, LP;HEMMINGSEN, SM
分子伴侣蛋白家族的分子伴侣存在于原核生物以及质体和线粒体中。蛋白质和线粒体伴侣蛋白-60寡聚体（Cpn-60）由单一亚基类型（p60 cpn-60）组成。相反，纯化的质体Cpn-60的制备物含有大约等量的两种多肽，p60 cpn-60 α。和p60 cpn-60 β，电泳迁移率略有不同。我们分离了编码质体p60 cpn-60 α的cDNA克隆。和p60 cpn-60 β。来自欧洲油菜和拟南芥的多肽。在p60 cpn-60 α之间观察到的出乎意料的序列差异程度。和p60 cpn-60 β。提出了关于寡聚体的结构和这些多肽的功能的问题。我们还在所有p60 cpn-60序列中发现了一个类似于p10 cpn-10序列的氨基酸序列基序。
Molecular chaperones of the chaperonin family occur in prokaryotes and in plastids and mitochondria. Prokaryotic and mitochondrial chaperonin-60 oligomers (Cpn-60) are composed of a single subunit type (p60cpn-60). In contrast, preparations of purified plastid Cpn-60 contain approximately equal quantities of two polypeptides, p60cpn-60.alpha. and p60cpn-60.beta., with slightly different electrophoretic mobilities. We have isolated cDNA clones encoding plastid p60cpn-60.alpha. and p60cpn-60.beta. polypeptides from Brassica napus and Arabidopsis thaliana. The unexpected degree of sequence divergence observed between p60cpn-60.alpha. and p60cpn-60.beta. raises questions concerning the structure of the oligomer and the functions of these polypeptides. We have also found an amino acid sequence motif within all p60cpn-60 sequences which resembles the p10cpn-10 sequences.