Time-lapse analysis reveals different modes of primordial germ cell migration in the medaka Oryzias latipes
Time-lapse analysis reveals different modes of primordial germ cell migration in the medaka Oryzias latipes
复制标题
DOI：
10.1111/j.1440-169x.2006.00858.x
复制
发表时间：
2006-04-01
影响因子：
2.5
通讯作者：
Tanaka, M
中科院分区：
文献类型：
--
作者：
Kurokawa, H;Aoki, Y;Tanaka, M
以往的研究表明，青鳉原始生殖细胞（PGC）是第一次区分由olvas表达在原肠胚形成后期，他们迁移到性腺区域通过侧板中胚层。在这里，我们证明青鳉nanos表达标志着在早期原肠胚形成阶段的生殖线。通过用与nanos 3'非翻译区融合的绿色荧光蛋白（GFP）标记生殖系，我们能够使用延时成像可视化PGC的行为。我们发现，有三种不同的PGC迁移模式，在不同的发展阶段发挥作用。在原肠胚形成早期，PGC主动向边缘区迁移，这一过程需要趋化因子受体CXCR 4的功能。然而，在原肠胚形成后期，PGC改变了它们的运动方式和方向，因为它们被带向中线沿着进行会聚运动的体细胞。在两侧对齐后，PGC主动迁移到侧板中胚层的后端。这种后移取决于HMGCoAR和CXCR 4的配体SDF-1a的活性。这些结果表明，PGC经历不同的迁移模式到达胚胎的预期性腺区域。
Previous studies have shown that medaka primordial germ cells (PGC) are first distinguishable by olvas expression during late gastrulation, and that they migrate to the gonadal region through the lateral plate mesoderm. Here, we demonstrate that medaka nanos expression marks the germ line at early gastrulation stage. By marking the germ line with green fluorescent protein (GFP) fused to the nanos 3' untranslated region, we were able to visualize the behavior of PGC using time-lapse imaging. We show that there are three distinct modes of PGC migration that function at different stages of development. At early gastrulation stage, PGC actively migrate towards the marginal zone, a process that requires the function of a chemokine receptor, CXCR4. However, at late gastrulation stage, PGC change the mode and direction of their movement, as they are carried towards the midline along with somatic cells undergoing convergent movements. After aligning bilaterally, PGC actively migrate to the posterior end of the lateral plate mesoderm. This posterior movement depends on the activity of both HMGCoAR and a ligand of CXCR4, SDF-1a. These results demonstrate that PGC undergo different modes of migration to reach the prospective gonadal region of the embryo.