Luteinizing Hormone Receptors Are Self-Associated in the Plasma Membrane* *This work was supported by NIH Grants HD-23236 and HD-01067 (to D.A.R.).
Luteinizing Hormone Receptors Are Self-Associated in the Plasma Membrane* *This work was supported by NIH Grants HD-23236 and HD-01067 (to D.A.R.).
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DOI：
10.1210/endo.141.12.7802
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发表时间：
2000-12
期刊：
影响因子：
4.8
通讯作者：
D. Roess;R. Horvat;H. Munnelly;B. Barisas
中科院分区：
文献类型：
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作者：
D. Roess;R. Horvat;H. Munnelly;B. Barisas
我们已经评估了大鼠LH受体的自我关联和横向动力学的功能和非功能性受体结合激素后。我们证明，第一次，在电子或光学显微镜下观察到的分组受体代表实际的受体二聚体或低聚体，而不仅仅是膜微区内的受体的浓度。条纹荧光光漂白恢复方法表明，无论是LH或人CG（hCG）的结合后，功能性野生型LH受体，293细胞（LHR-wt细胞）上表达，具有的迁移率是25%低于那些非功能性LH受体含有精氨酸取代赖氨酸在位置583（LHR-K583 R细胞）。由于在两个维度的横向扩散系数仅取决于扩散物种的分子大小的对数，这一结果意味着功能性受体存在于比非功能性受体大得多的膜复合物中。在荧光能量的单细胞测量中...
We have evaluated rat LH receptor self-association and lateral dynamics for functional and nonfunctional receptors after binding of hormone. We demonstrate, for the first time, that grouped receptors observed in electron or light microscopy represent actual receptor dimers or oligomers rather than simply the concentration of receptors within membrane microdomains. Fringe fluorescence photobleaching recovery methods showed that, after binding of either LH or human CG (hCG), functional wild-type LH receptors, expressed on 293 cells (LHR-wt cells), have mobilities that are 25% lower than those of nonfunctional LH receptors containing an arginine substitution for lysine at position 583 (LHR-K583R cells). Because lateral diffusion coefficients in two dimensions depend only on the logarithm of the molecular size of the diffusing species, this result implies that functional receptors exist in substantially larger membrane complexes than do nonfunctional receptors. In single-cell measurements of fluorescence ener...