Temporal-spatial patterns of ABCDE model genes expressed in new flowers that emerge from the centres of existing flowers in Chinese cabbage
Temporal-spatial patterns of ABCDE model genes expressed in new flowers that emerge from the centres of existing flowers in Chinese cabbage
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大白菜现有花中心出现的新花中ABCDE模型基因表达的时空模式
DOI：
10.1080/14620316.2017.1361341
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发表时间：
2018
影响因子：
1.9
通讯作者：
Shen Shu Xing
中科院分区：
文献类型：
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作者：
Gu Ai Xia;Meng Chuan;Chen Yue Qi;Xuan Shu Xin;Li Xiao Feng;Luo Shuan Xia;Zhao Yu Jing;Feng Da Ling;Zhang Wei Wei;Shen Shu Xing
我们研究了五个生长阶段的花芽和花器官的形成，并比较了正常花和从另一朵花的中心出现的花（即花中花）。通过qRT-PCR分析基因表达。当与正常花比较时，9个基因在相应时间段的表达在花中花样品中显示出明显不同的趋势，这取决于这些异常结构是第一类型（1-1和1-2）还是第二类型（2-1和2-2）。例如，在每个时间点，AP 2、STK和SHP在2-1朵花中的表达显著高于1-1朵或1-2朵花。相比之下，AG表达上调之间的阶段4和5正常的花比其他类型。此外，与正常花器官相比，突变体样品的雄蕊和雌蕊中几乎检测不到AP 1转录本，花中花的萼片和花瓣中的表达量较低。除第5期2-2朵花外，STK和SHP在突变体器官中的表达均高于正常器官。这些结果表明，花中花形态的形成与AP 1和AG的下调以及AP 2、STK和SHP的上调密切相关。
We examined the formation of flower buds and floral organs at five growth stages and compared between normal flowers and those that emerged from the centre of another flower (i.e. flowers-in-flower). Gene expression was analysed via qRT-PCR. When compared with normal flowers, expression of nine genes at corresponding time periods showed clearly different trends in the flowers-in-flower samples, depending upon whether those abnormal structures were of the first types (1–1 and 1–2) or the second types (2–1 and 2–2). For example, at each time point, expression ofAP2,STKandSHPwas notably higher in 2–1 flowers than in either 1–1 or 1–2 flowers. By contrast,AGexpression was up-regulated more between Stages 4 and 5 for normal flowers than for the other types. Furthermore, when compared with normal floral organs,AP1transcripts were barely detected in the stamens and pistils of mutant samples, and expression was lower in the flowers-in-flower sepals and petals. Except for 2–2 flowers at Stage 5,STKandSHPexpression was higher in the mutant organs than in the normal organs. These results suggest that formation of the flowers-in-flower morphology is closely related to down-regulation ofAP1andAGand up-regulation ofAP2,STK, andSHP.