Transfection of Staphylococcus aureus with Bacteriophage Deoxyribonucleic Acid
Transfection of Staphylococcus aureus with Bacteriophage Deoxyribonucleic Acid
复制标题
噬菌体脱氧核糖核酸转染金黄色葡萄球菌
DOI：
--
复制
发表时间：
1972
影响因子：
3.2
通讯作者：
L. Philipson
中科院分区：
文献类型：
--
作者：
J. Sjöström;M. Lindberg;L. Philipson
菌株8325 (N)的金黄色葡萄球菌细胞在早期指数生长阶段收获时能够吸收噬菌体脱氧核糖核酸（DNA）。能力的表型表达需要二价阳离子，而钙离子是最有效的。用脱氧核糖核酸酶处理噬菌体DNA完全破坏感染性，热变性DNA不具有传染性。转染的最高频率约为每μg DNA 104个斑块形成单位。
Staphylococcus aureus cells of strain 8325 (N) are competent for phage deoxyribonucleic acid (DNA) when harvested in the early exponential growth phase. Phenotypic expression of the competence requires divalent cations, and calcium ions are most effective. Treatment of phage DNA with deoxyribonuclease completely destroys infectivity and heat-denaturated DNA is not infectious. The highest frequency of transfection is around 104 plaque-forming units per μg of DNA.