Conductive Polymer-Coated 3D Printed Microneedles: Biocompatible Platforms for Minimally Invasive Biosensing Interfaces.
Conductive Polymer-Coated 3D Printed Microneedles: Biocompatible Platforms for Minimally Invasive Biosensing Interfaces.
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DOI：
10.1002/smll.202206301
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发表时间：
2023-01
期刊：
影响因子：
13.3
通讯作者：
Antonios Keirouz;Yasemin L. Mustafa;Joseph G. Turner;E. Lay;Ute Jungwirth;F. Marken;H. Leese
中科院分区：
文献类型：
--
作者：
Antonios Keirouz;Yasemin L. Mustafa;Joseph G. Turner;E. Lay;Ute Jungwirth;F. Marken;H. Leese
导电聚合物微针（MN）阵列作为生物界面材料显示出对生物设备和可穿戴设备中分析物的微创监测的前景。人们对微针作为生物传感电极的兴趣越来越大，但努力仅限于金属基底，其缺乏生物稳定性，并且与高制造成本和费力的制造方法相关，这会产生平移障碍。在这项工作中，增材制造，这为用户提供了设计灵活性和高档制造，被用来制造丙烯酸基微针装置。这些微针装置用作平台以产生基于聚吡咯（PPy）和聚（3，4-亚乙基二氧噻吩）-聚（苯乙烯磺酸盐）（PEDOT：PSS）的本质导电的聚合物基表面。这些完全基于聚合物的固体微针阵列用作干导电电极，同时省略了对金属种子层的要求。3D打印固体微针的两种不同的涂覆方法，原位聚合和滴铸，使导电功能成为可能。微针阵列穿透离体猪皮肤移植物，而不影响导电性或微针形态，并证明涂层在多次穿透循环中的耐久性。使用人成纤维细胞评价导电微针的无细胞毒性性质。所提出的制造策略提供了一种引人注目的方法来制造基于聚合物的导电微针表面，可以进一步利用作为生物传感的平台。
Conductive polymeric microneedle (MN) arrays as biointerface materials show promise for the minimally invasive monitoring of analytes in biodevices and wearables. There is increasing interest in microneedles as electrodes for biosensing, but efforts have been limited to metallic substrates, which lack biological stability and are associated with high manufacturing costs and laborious fabrication methods, which create translational barriers. In this work, additive manufacturing, which provides the user with design flexibility and upscale manufacturing, is employed to fabricate acrylic-based microneedle devices. These microneedle devices are used as platforms to produce intrinsically-conductive, polymer-based surfaces based on polypyrrole (PPy) and poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT:PSS). These entirely polymer-based solid microneedle arrays act as dry conductive electrodes while omitting the requirement of a metallic seed layer. Two distinct coating methods of 3D-printed solid microneedles, in situ polymerization and drop casting, enable conductive functionality. The microneedle arrays penetrate ex vivo porcine skin grafts without compromising conductivity or microneedle morphology and demonstrate coating durability over multiple penetration cycles. The non-cytotoxic nature of the conductive microneedles is evaluated using human fibroblast cells. The proposed fabrication strategy offers a compelling approach to manufacturing polymer-based conductive microneedle surfaces that can be further exploited as platforms for biosensing.