BIOASSAY FOR STUDYING THE ROLE OF SIDEROPHORES IN POTATO GROWTH STIMULATION BY PSEUDOMONAS SPP IN SHORT POTATO ROTATIONS
BIOASSAY FOR STUDYING THE ROLE OF SIDEROPHORES IN POTATO GROWTH STIMULATION BY PSEUDOMONAS SPP IN SHORT POTATO ROTATIONS
复制标题
研究铁载体在马铃薯短轮作中假单胞菌刺激马铃薯生长中的作用的生物测定
DOI：
10.1016/0038-0717(87)90036-8
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发表时间：
1987
影响因子：
9.7
通讯作者：
B. Schippers
中科院分区：
文献类型：
--
作者：
P. Bakker;A. Bakker;J. Marugg;P. Weisbeek;B. Schippers
本文报道了一种用马铃薯插条研究荧光假单胞菌促生长机理的生物测定法。与长期轮作土壤相比，短期轮作土壤对马铃薯插条根系发育有抑制作用。荧光假单胞菌分离物WCS374或恶臭假单胞菌分离物WCS358处理增加了短马铃薯轮作土壤中的根发育，而铁载体阴性Tn5转座子突变体分离物WCS358没有影响。无论是铁载体生产和铁载体阴性Tn5突变体的隔离WCS358可以回收到类似的数量从根系。这些结果是在8天的生物测定中获得的。据推测，分离物WCS358的铁载体阴性Tn5转座子突变体诱导生长刺激的失败表明，铁载体的产生是在短马铃薯轮作土壤中生长刺激的先决条件。
A bioassay is described for studying the mechanisms of growth stimulation by fluorescent pseudomonads using rooted potato stem cuttings. Root development of potato stem cuttings was inhibited in short potato-rotation soil compared to development in long potato-rotation soil. Treatment withPseudomonas fluorescensisolate WCS374 orPseudomonas putidaisolate WCS358 increased root development in short potato-rotation soil, whereas siderophore-negative Tn5 transposon mutants of isolate WCS358 had no effect. Both siderophore-producing and siderophore-negative Tn5 mutants of isolate WCS358 could be recovered in similar numbers from the root systems. These results were obtained in a bioassay of 8 days duration.It is postulated that failure of siderophore-negative Tn5 transposon mutants of isolate WCS358 to induce growth stimulation demonstrates that siderophore production is a prerequisite for growth stimulation in short potato-rotation soil.