Effects of aging and exercise training on the histological and mechanical properties of articular structures in knee joints of male rat
Effects of aging and exercise training on the histological and mechanical properties of articular structures in knee joints of male rat
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DOI：
10.1007/s10522-012-9381-8
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发表时间：
2012-04
期刊：
影响因子：
4.5
通讯作者：
H. Moriyama;N. Kanemura;I. Brouns;I. Pintelon;D. Adriaensen;J. Timmermans;J. Ozawa;N. Kito;T. Gomi;M. Deie
中科院分区：
文献类型：
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作者：
H. Moriyama;N. Kanemura;I. Brouns;I. Pintelon;D. Adriaensen;J. Timmermans;J. Ozawa;N. Kito;T. Gomi;M. Deie
衰老对关节的影响会对个人的功能产生深远的影响。我们的目的是评估随年龄增长的膝关节囊和关节软骨的组织学和力学特性，并检查运动对膝关节年龄相关变化的影响。2岁的Wistar大鼠被分为久坐对照组和运动训练组。10周大的动物被用来研究随年龄增长的变化。用组织学、组织形态学、免疫组织化学和力学分析对关节囊和软骨进行评估。随着年龄的增长，观察到关节软骨发生了严重的退行性变化，而运动显然没有显著的影响。老龄大鼠关节软骨表现为软骨表面损伤、细胞聚集、软骨基质异常。组织形态学分析进一步显示软骨厚度的变化以及软骨细胞数量的减少。老化导致关节软骨僵硬，降低了关节内产生的分散载荷和分配应变的能力。关节僵硬与老化无关，关节囊僵硬和滑膜炎不是老化关节的特征。这项研究证实，在大鼠模型中，衰老本身最终会导致关节变性。老化的关节变化缺乏恢复可能是由于几个因素，如干预的持续时间和软骨的再生能力。
The impact of aging on joints can have a profound effect on an individual’s functioning. Our objectives were to assess the histological and mechanical properties of the knee joint capsule and articular cartilage with aging, and to examine the effects of exercise on age-related changes in the knee joint. 2-year-old Wistar rats were divided into a sedentary control group and an exercise-trained group. 10-week-old animals were used to investigate the changes with aging. The joint capsule and cartilage were evaluated with histological, histomorphometric, immunohistochemical, and mechanical analyses. Severe degenerative changes in articular cartilage were observed with aging, whereas exercise apparently did not have a significant effect. The articular cartilage of aged rats was characterized by damage to the cartilage surface, cell clustering, and an abnormal cartilage matrix. Histomorphometric analysis further revealed changes in cartilage thickness as well as a decreased number of chondrocytes. Aging led to stiffness of the articular cartilage and reduced the ability to dissipate the load and distribute the strain generated within the joint. Joint stiffness with aging was independent of capsular stiffness and synovitis was not a characteristic feature of the aging joint. This study confirms that aging alone eventually leads to joint degeneration in a rat model. The lack of recovery in aging joint changes may be due to several factors, such as the duration of the intervention and the regeneration ability of the cartilage.