Oriented nucleation of hydroxylapatite crystals on spider dragline silks
Oriented nucleation of hydroxylapatite crystals on spider dragline silks
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DOI：
10.1021/la7014435
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发表时间：
2007-10-09
期刊：
影响因子：
3.9
通讯作者：
Mao, Chuanbin
中科院分区：
文献类型：
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作者：
Cao, Binrui;Mao, Chuanbin
蜘蛛拖丝作为一种蛋白质纤维可以被描绘为蛋白质纳米晶体沿着长轴的定向组织，其间距被无定形蛋白质结构域填充。我们使用蜘蛛拖丝的表面作为生物模板，从羟基磷灰石（HAP）过饱和溶液中使骨矿物HAP位点特异性地成核。发现HAP晶体在丝表面上成核，其c轴相对于丝的长轴以平均72.9度的角度优先取向。我们研究的不同矿化丝之间的择优取向几乎是相同的。其他材料如Au和CdS可以在丝上成核，但没有显示出任何择优取向。我们认为HAP的取向成核与丝素和HAP的结构有直接关系。矿化丝将联合收割机结合蜘蛛丝良好的力学性能和羟基磷灰石的生物相容性，可组装成理想的骨植入材料。
Spider dragline silk as a protein fiber can be pictured as the oriented organization of protein nanocrystals along the long axis with their spacing filled by amorphous protein domains. We used the surface of the spider dragline silk as a biological template to nucleate bone mineral hydroxylapatite (HAP) site-specifically from a HAP-supersaturated solution. HAP crystals were found to be nucleated on the surface of silks with their c axis preferentially oriented at an average angle of 72.9 degrees with respect to the long axis of the silks. The preferred orientation is nearly identical among the different mineralized silks that we studied. Other materials such as Au and CdS could be nucleated on the silks but did not show any preferred orientation. We believe that the oriented nucleation of HAP is directly related to the structures of silks and HAP. The mineralized silks will combine the good mechanical properties of the spider silks and the biocompatibility of HAP and may be assembled into ideal biomaterials as bone implants.