BALB/c mice can be used to evaluate allergenicity of different food protein extracts
BALB/c mice can be used to evaluate allergenicity of different food protein extracts
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BALB/c小鼠可用于评估不同食物蛋白提取物的致敏性
DOI：
10.1080/09540105.2015.1129600
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发表时间：
2016-09-01
影响因子：
3
通讯作者：
Che, Huilian
中科院分区：
文献类型：
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作者：
Zhou, Cui;Ludmila, Tekutyeva;Che, Huilian
摘要本研究旨在验证BALB/c小鼠是一种有用的过敏模型，然后研究不同食物的过敏性。使用与初步研究相同的方案，小鼠暴露于花生蛋白提取物(PPE)、脱脂牛奶提取物或土豆蛋白提取物。分析过敏原特异性IgG1和IgE、血液中的组胺、肥大细胞、脾和其他组织中的亚群分布和细胞因子，以及细胞因子的形成。结果表明，在接受PPE的小鼠中，血清组胺和抗原特异性IgE水平最高(与对照组相比)。这些小鼠也有最多的肥大细胞，TH2细胞，但最低的Treg细胞。PPE过敏患者血清中变应原特异性IgE/IgG4比值高于牛奶过敏患者，这是为了解释这些结果。上述结果证实了BALB/c小鼠是评价不同食物过敏性的合适模型。
ABSTRACT The present study sought to verify BALB/c mice as a useful allergy model and to then investigate allergenicities of different foods. Using the same regimens as preliminary study, mice were exposed to peanut protein extract (PPE), de-fatted cow milk extract, or potato protein extract. Allergen-specific IgG1 and IgE, histamine in blood, mast cell, sub-population profiles and cytokine in spleen and other tissues, and cytokine formation were analyzed. The results showed that serum levels of histamine and antigen-specific IgE were greatest (vs. control) among mice that received PPE. These mice also had the greatest presence of mast cells, TH2 cells, but lowest Treg cells. Ratios of allergen-specific IgE/IgG4 in the sera of PPE-allergic patients were higher than that in milk-allergic patients, which was performed to explain these outcomes. All of the above results confirmed that BALB/c mice is a suitable model to evaluate allergenicity of different food.