Curvature sensing by the epsin N-terminal homology domain measured on cylindrical lipid membrane tethers.
Curvature sensing by the epsin N-terminal homology domain measured on cylindrical lipid membrane tethers.
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DOI：
10.1021/ja907936c
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发表时间：
2010-02-03
影响因子：
15
通讯作者：
Baumgart, Tobias
中科院分区：
文献类型：
--
作者：
Capraro, Benjamin R.;Yoon, Youngdae;Cho, Wonhwa;Baumgart, Tobias
epsin蛋白被认为在网格蛋白介导的内吞作用中起重要作用，包括囊泡形成所需的高膜曲率的产生。本文通过系统地量化epsin n端同源（ENTH）结构域在曲率可控的圆柱形膜上的面积密度对曲率的依赖性来评估这一假设的基础。荧光显微镜观察到，从微管抽吸的巨大单层囊泡中拉出的圆柱形系绳中，膜结合的ENTH从囊泡重新分配到高度弯曲的膜上。一阶热力学理论用于分析我们的数据产生了第一个测量Leibler的热力学曲率-组成耦合系数的内吞噬辅助蛋白。我们的结果强调了epsin有助于细胞膜曲率感知和产生的可能性，我们相信我们的方法将为将相互作用的分子描述与细胞宏观膜重塑以及识别和表征这些过程中蛋白质的作用相关的目标提供有用的贡献。
The protein epsin is believed to play important roles in clathrin-mediated endocytosis, including generation of the high membrane curvature necessary for vesicle formation. Here we assess the basis for this hypothesis by systematically quantifying the curvature dependence of the area density of epsin N-terminal homology (ENTH) domain on cylindrical membranes of controlled curvature. In cylindrical tethers pulled from micropipet-aspirated giant unilamellar vesicles, repartitioning of membrane-bound ENTH from vesicles onto highly curved membranes was observed by fluorescence microscopy. First-order thermodynamic theory used to analyze our data yielded the first measurement of Leibler’s thermodynamic curvature−composition coupling coefficient to be reported for an endocytic accessory protein. Our results highlight the possibility that epsin contributes to cellular membrane curvature sensing and generation, and we believe that our method will provide useful contributions toward the goal of relating molecular descriptions of interactions to macroscopic membrane remodeling in cells and identifying and characterizing roles for proteins in these processes.
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影响因子：
4
作者：
Legendre-Guillemin, V;Wasiak, S;McPherson, PS
通讯作者：
McPherson, PS
影响因子：
64.8
作者：
Ford, MGJ;Mills, IG;McMahon, HT
通讯作者：
McMahon, HT
影响因子：
5.6
作者：
Hom, Robert A.;Vora, Mohsin;Kutateladze, Tatiana G.
通讯作者：
Kutateladze, Tatiana G.
DOI：
10.1051/jphys:01986004703050700
发表时间：
1986-03-01
期刊：
JOURNAL DE PHYSIQUE
影响因子：
--
作者：
LEIBLER, S
通讯作者：
LEIBLER, S
影响因子：
56.9
作者：
Itoh, T;Koshiba, S;Takenawa, T
通讯作者：
Takenawa, T