Pathological Study of Acute Pulmonary Toxicity Induced by Intratracheally Instilled Asian Sand Dust (Kosa)
Pathological Study of Acute Pulmonary Toxicity Induced by Intratracheally Instilled Asian Sand Dust (Kosa)
复制标题
DOI：
10.1177/0192623310385143
复制
发表时间：
2010-12-01
影响因子：
1.5
通讯作者：
Takano, Hirohisa
中科院分区：
文献类型：
--
作者：
Naota, Misaki;Mukaiyama, Toru;Takano, Hirohisa
本研究旨在探讨亚洲沙尘对小鼠的急性肺毒性作用。使用了从中国丁格尔沙漠采集的模拟亚洲沙尘(CJ-2颗粒)和从日本大气采集的亚洲沙尘(鸟取颗粒)。将50、200、800和3000毫克亚洲沙尘的生理盐水悬液分别注入ICR小鼠气管内。肺组织的细支气管壁和肺泡内可见局限性的粉尘颗粒聚集，颗粒周围可见以巨噬细胞和中性粒细胞浸润为特征的急性炎症改变。观察到肺泡壁和支气管上皮细胞的退变以及层粘连蛋白免疫标记的减弱与颗粒附着有关。白介素6、肿瘤坏死因子-α诱导型一氧化氮合酶和含铜锌二聚体超氧化物歧化酶的阳性免疫标记主要见于病变内的炎性细胞中，而对照组和缺损区则未见。这些结果表明，亚洲沙尘颗粒通过直接的物理效应对肺组织造成损害。此外，损伤处产生的二次释放的细胞因子和氧化应激可能参与了急性肺毒性的发生。
The objective of this study was to investigate acute lung toxicity caused by Asian sand dust. Simulated Asian sand dust collected from the Tennger desert in China (CJ-2 particles) and Asian sand dust collected from the atmosphere in Japan (Tottori particles) were used. Saline suspensions of 50, 200, 800, and 3,000 mu g Asian sand dust were intratracheally instilled to ICR mice. Localized accumulation of the dust particles was observed in the bronchioles and the alveoli of the lung tissues; acute inflammatory changes characterized by infiltration of macrophages and neutrophils were observed around the particles. Degenerated alveolar walls and bronchial epithelial cells, as well as a weakened positive immunolabeling for laminin, were observed to be associated with particle attachment. Positive immunolabelings for interleukin-6, tumor necrosis factor-alpha inducible nitric oxide synthase, and dimeric copper-and zinc-containing superoxide dismutase were observed mainly in the inflammatory cells in the lesions; these findings were not observed in the controls or in areas lacking lesions. These results suggest that Asian sand dust particles caused damage to the lung tissue through a direct physical effect. In addition, secondary released cytokines and oxidative stress generated in the lesion may be involved in the development of the acute lung toxicity.