Mapping Antiretroviral Drugs in Tissue by IR-MALDESI MSI Coupled to the Q Exactive and Comparison with LC-MS/MS SRM Assay
Mapping Antiretroviral Drugs in Tissue by IR-MALDESI MSI Coupled to the Q Exactive and Comparison with LC-MS/MS SRM Assay
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DOI：
10.1007/s13361-014-0884-1
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发表时间：
2014-12-01
影响因子：
3.2
通讯作者：
Muddiman, David C.
中科院分区：
文献类型：
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作者：
Barry, Jeremy A.;Robichaud, Guillaume;Muddiman, David C.
这项工作描述了IR-MALDESI成像源与Q Exactive质谱仪的耦合。IR-MALDESI MSI用于阐明几种HIV药物在宫颈组织中的空间分布，这些药物以低浓度或高浓度孵育。将通过IR-MALDESI MSI分析的连续切片均质化并通过LC-MS/MS分析以定量组织中存在的每种药物的量。通过比较这两种技术，观察到来自成像实验的平均强度与每种药物的绝对量之间的一致性。这两种技术之间的这种相关性是定量IR-MALDESI MSI的先决条件。此外，还进行了一项目标MS 2成像实验，以证明Q Exactive的能力，并突出显示SRM或MRM成像实验可获得的额外选择性。
This work describes the coupling of the IR-MALDESI imaging source with the Q Exactive mass spectrometer. IR-MALDESI MSI was used to elucidate the spatial distribution of several HIV drugs in cervical tissues that had been incubated in either a low or high concentration. Serial sections of those analyzed by IR-MALDESI MSI were homogenized and analyzed by LC-MS/MS to quantify the amount of each drug present in the tissue. By comparing the two techniques, an agreement between the average intensities from the imaging experiment and the absolute quantities for each drug was observed. This correlation between these two techniques serves as a prerequisite to quantitative IR-MALDESI MSI. In addition, a targeted MS2 imaging experiment was also conducted to demonstrate the capabilities of the Q Exactive and to highlight the added selectivity that can be obtained with SRM or MRM imaging experiments.