Accelerated Polymer-Polymer Click Conjugation by Freeze-Thaw Treatment
Accelerated Polymer-Polymer Click Conjugation by Freeze-Thaw Treatment
复制标题
通过冻融处理加速聚合物-聚合物点击结合
DOI：
10.1021/bc300182y
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
Kazunori Kataoka
中科院分区：
文献类型：
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作者：
Hiroyasu Takeraoto;Kanjiro Miyata;Shota Hattori;Shigehito Osawa;Nobuhiro Nishiyama;Kazunori Kataoka
在此，我们报告了一种基于无铜点击化学的加速聚合物-SNA缀合的独特技术：反应溶液的逐渐冻融显著增加了缀合速率，这可能是因为在微环境尺度下的反应物浓度。将该技术应用于在室温/低于室温的水性缓冲液中小干扰RNA（siRNA）和PEG之间的缀合。
Herein, we report a unique technique to accelerate polymer–SNA conjugation based on copper-free click chemistry: gradual freeze–thawing of the reaction solution substantially increases the conjugation rate possibly because of the reactant concentration at the microenvironment scale. This technique was applied to the conjugation between a small interfering RNA (siRNA) and PEG in an aqueous buffer at/below room temperature.