NaCl Induces Flavonoid Biosynthesis through a Putative Novel Pathway in Post-harvest Ginkgo Leaves.
NaCl Induces Flavonoid Biosynthesis through a Putative Novel Pathway in Post-harvest Ginkgo Leaves.
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氯化钠通过收获后银杏叶中假定的新途径诱导类黄酮生物合成
DOI：
10.3389/fpls.2017.00920
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发表时间：
2017
影响因子：
5.6
通讯作者：
Xu M
中科院分区：
文献类型：
--
作者：
Ni J;Hao J;Jiang Z;Zhan X;Dong L;Yang X;Sun Z;Xu W;Wang Z;Xu M
银杏叶提取物中的黄酮类化合物具有很高的药用价值，因此，提高银杏叶提取物中黄酮类化合物的含量对人体健康具有重要意义。在本研究中，我们研究了不同处理对采后银杏叶片中类黄酮含量及相应基因表达水平的影响。我们发现，紫外线-B和氯化钠处理都诱导了类黄酮的积累。然而，基因表达分析表明，类黄酮含量的增加是通过不同的途径实现的。此外，采后银杏叶片对氯化钠处理的反应与自然生长的叶片相比，无论是类黄酮含量还是相应的基因表达都有所不同。此外，紫外线B和氯化钠联合处理与单独使用紫外线B或氯化钠处理相比，并没有进一步增加总黄酮的含量。我们的结果表明，采后银杏叶片存在一种新的响应机制，并为提高制药行业中的类黄酮含量提供了一种技术。
The flavonoids in the extracts of Ginkgo leaves have been shown to have great medical value: thus, a method to increase the flavonoid contents in these extracts is of significant importance for human health. In the present study, we investigated the changes in flavonoid contents and the corresponding gene expression levels in post-harvest Ginkgo leaves after various treatments. We found that both ultraviolet-B and NaCl treatment induced flavonoid accumulation. However, gene expression analysis showed that the increases in flavonoid contents were achieved by different pathways. Furthermore, post-harvest Ginkgo leaves responded differently to NaCl treatment compared with naturally grown leaves in both flavonoid contents and corresponding gene expression. In addition, combined treatment with ultraviolet-B and NaCl did not further increase the flavonoid contents compared with ultraviolet-B or NaCl treatment alone. Our results indicate the existence of a novel mechanism in response to NaCl treatment in post-harvest Ginkgo leaves, and provide a technique to increase flavonoid content in the pharmaceutical industry.