The late effects of radiation on lifespan, lymphocyte proliferation and p53 haplodeficiency in mice
The late effects of radiation on lifespan, lymphocyte proliferation and p53 haplodeficiency in mice
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DOI：
10.3109/09553002.2010.496031
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发表时间：
2010-11-01
影响因子：
2.6
通讯作者：
Norimura, Toshiyuki
中科院分区：
文献类型：
--
作者：
Okazaki, Ryuji;Mabuchi, Yo;Norimura, Toshiyuki
目的：方法：用3戈伊剂量照射8周龄小鼠，观察不同剂量照射对小鼠寿命、骨髓淋巴细胞数和脾细胞p53蛋白表达水平的影响。流式细胞仪检测骨髓细胞数和细胞周期时相。通过荧光细胞活力测定评价脾细胞增殖。结果：照射组小鼠寿命较对照组缩短，而对照组小鼠寿命较照射组缩短。在照射的72周龄p53（+/+）小鼠和56周龄p53（+/-）小鼠中，与未照射小鼠相比，骨髓中的淋巴细胞数量减少。在56周龄p53（+/-）小鼠中，与未照射小鼠相比，照射小鼠中淋巴细胞的S-和G2/M-期增加。p53（+/+）小鼠脾细胞增殖随年龄增长而降低，照射组的增殖明显低于未照射组。72周龄p53（+/+）小鼠再照射后，受照小鼠脾细胞p53蛋白表达较未受照小鼠延迟。结论：受照小鼠骨髓淋巴细胞数量减少和脾细胞p53蛋白表达延迟与受照小鼠寿命缩短有关。
Purpose: We investigated the effect of irradiation on the lifespan of eight-week-old mice, the number of lymphocytes in bone marrow and the levels of p53 protein expression in the splenocytes.Methods and materials: Eight-week-old mice, wild-type p53 (p53(+/+)) and heterozygous p53 (p53(+/-)), were irradiated with 3 Gy. The cell numbers and cell cycle phases of bone marrow cells were determined by flow cytometry. The splenocyte proliferation was evaluated by a fluorescent cell viability assay. The p53 expression was evaluated by Western blotting.Results: The lifespan of the irradiated mice was shorter than that of the non-irradiated mice. In irradiated 72-week-old p53(+/+) mice and 56-week-old p53(+/-) mice, the number of lymphocytes in bone marrow decreased as compared to that in the non-irradiated mice. In 56-week-old p53(+/-) mice, the S- and G2/M-phases of lymphocytes in the irradiated mice were increased compared to that in the non-irradiated mice. The splenocyte proliferation in p53(+/+) mice decreased with age, and the proliferation in the irradiated mice was much lower than that in the non-irradiated mice. In 72-week-old p53(+/+) mice after re-irradiation, the p53 protein expression in the splenocytes of the irradiated mice was delayed as compared to those from the non-irradiated mice.Conclusion: We suggest that the decrease in the number of lymphocytes in bone marrow and the delayed p53 expression in splenocytes from the irradiated mice are related to the shortened lifespan after irradiation at a young age.