Patterns of microbial community biomass, composition and HPLC diagnostic pigments in the Costa Rica upwelling dome.
Patterns of microbial community biomass, composition and HPLC diagnostic pigments in the Costa Rica upwelling dome.
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哥斯达黎加上升流圆顶微生物群落生物量、组成和 HPLC 诊断色素的模式。
DOI：
10.1093/plankt/fbv086
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发表时间：
2016
影响因子：
2.1
通讯作者：
A. Gutiérrez‐Rodríguez
中科院分区：
文献类型：
--
作者：
Andrew G. Taylor;M. Landry;A. Freibott;K. Selph;A. Gutiérrez‐Rodríguez
我们于 2010 年 6 月至 7 月调查了哥斯达黎加穹顶 (CRD) 浮游植物群落的生物量、大小结构、组成、深度分布和空间变异性。在穹顶区域内外的拉格朗日实验期间，每天对透光区剖面进行采样，并结合使用数字落射荧光显微镜、流式细胞术和 HPLC 颜料对群落进行分析。在跟踪 4 天的 4 个水块中，浮游植物的平均深度综合生物量范围为 2 倍，从 1089 到 1858 mg C m-2 （平均值 ± SE = 1378 ± 112 mg C m-2）。总叶绿素 a (Chl a) 的相应平均值 (±SE) 积分值和自养碳与 Chl a 的比率分别为 24.1 ± 1.5 mg Chl a m-2 和 57.5 ± 3.4。微型浮游植物（∼60%）、聚球藻（>33%）和原绿球藻（17%）对浮游植物群落生物量的绝对和相对贡献在中央穹顶区域最高，而>20微米的浮游植物占所有区域生物量的≤10%，硅藻<2%。尽管如此，以甲藻为主的自养鞭毛虫在所有地点的生物量贡献都超过了聚球藻。基于诊断色素相对于显微镜的硅藻（高估）和甲藻（低估）的相对贡献的数量级差异突出了与从色素进行群落推论相关的潜在显着偏差。
We investigated biomass, size-structure, composition, depth distributions and spatial variability of the phytoplankton community in the Costa Rica Dome (CRD) in June-July 2010. Euphotic zone profiles were sampled daily during Lagrangian experiments in and out of the dome region, and the community was analyzed using a combination of digital epifluorescence microscopy, flow cytometry and HPLC pigments. The mean depth-integrated biomass of phytoplankton ranged 2-fold, from 1089 to 1858 mg C m-2 (mean ± SE = 1378 ± 112 mg C m-2), among 4 water parcels tracked for 4 days. Corresponding mean (±SE) integrated values for total chlorophyll a (Chl a) and the ratio of autotrophic carbon to Chl a were 24.1 ± 1.5 mg Chl a m-2 and 57.5 ± 3.4, respectively. Absolute and relative contributions of picophytoplankton (∼60%), Synechococcus (>33%) and Prochlorococcus (17%) to phytoplankton community biomass were highest in the central dome region, while >20 µm phytoplankton accounted for ≤10%, and diatoms <2%, of biomass in all areas. Nonetheless, autotrophic flagellates, dominated by dinoflagellates, exceeded biomass contributions of Synechococcus at all locations. Order-of-magnitude discrepancies in the relative contributions of diatoms (overestimated) and dinoflagellates (underestimated) based on diagnostic pigments relative to microscopy highlight potential significant biases associated with making community inferences from pigments.
DOI：
10.1016/j.dsr2.2010.08.005
发表时间：
2011-02-01
影响因子：
3
作者：
Brzezinski, Mark A.;Baines, Stephen B.;Twining, Benjamin S.
通讯作者：
Twining, Benjamin S.