Oxygen Damage to Bull Spermatozoa and its Prevention by Catalase
Oxygen Damage to Bull Spermatozoa and its Prevention by Catalase
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公牛精子的氧损伤及其过氧化氢酶的防治
DOI：
10.3168/jds.s0022-0302(49)92049-4
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发表时间：
1949
影响因子：
3.5
通讯作者：
R. Bratton
中科院分区：
文献类型：
--
作者：
N. Vandemark;G. Salisbury;R. Bratton
1.研究了牛精子在46.5℃的蛋黄柠檬酸盐稀释液中孵育和5℃下的保存，在空气、氧气和氮气中，空气和氧气中添加和不添加过氧化氢酶，以及在最低限度和常规混合过程中添加和不添加过氧化氢酶的情况下牛精子的活力。2.氧气产生有害影响，表现为精子的活力和存活率降低，将糖转化为乳酸的能力降低。3.稀释液中加入过氧化氢酶可基本消除氧对精子的损伤。4.温和混合缩短了蛋黄-柠檬酸稀释液中保存的公牛精子的寿命，但混合样品中添加过氧化氢酶的存在消除了混合的有害影响。5.在蛋黄-柠檬酸盐稀释剂中，过氧化氢酶能有效地防止氧气对公牛精子的有害作用和抖动，从而得出结论：增加曝气量和较高的氧分压会加快精子产生过氧化氢的速度，进而产生有毒的环境，缩短精子的寿命。6.结果表明，在常规人工繁殖中储存和运输的稀释精液上方应该留出最小的空间，精液样本应该进行最小程度的混合。
Summary 1. Bovine spermatozoan activity was studied in the egg yolk-citrate diluent during incubation at 46.5° C. and storage at 5° C. under air, oxygen and nitrogen, under air and oxygen with and without added catalase, and during storage when subjected to minimum and routine mixing procedures with and without added catalase. 2. Oxygen produced deleterious effects which were manifested by decreased motility and livability of the spermatozoa and a reduction in their ability to convert sugar to lactic acid. 3. Oxygen damage to spermatozoa was largely eliminated when catalase was added to the diluted semen. 4. Gentle mixing shortened the life of stored bull spermatozoa in the yolk-citrate diluent, but the presence of added catalase in mixed samples obliterated the harmful effects of mixing. 5. The effectiveness of catalase in preventing the harmful action of oxygen and shaking on bull spermatozoa in the egg yolk-citrate diluent led to the conclusion that increased aeration and higher oxygen tensions speed the production of hydrogen peroxide by the spermatozoa and this in turn produces a toxic environment, shortening the life of the spermatozoa. 6. The results suggest that a minimum of air space be left above the diluted semen that is stored and shipped in routine artificial breeding and that semen samples should be subjected to a minimum of mixing.