The effect of prostaglandin E2 on cystine uptake and glutathione synthesis by human lung fibroblasts.
The effect of prostaglandin E2 on cystine uptake and glutathione synthesis by human lung fibroblasts.
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前列腺素 E2 对人肺成纤维细胞胱氨酸摄取和谷胱甘肽合成的影响。
DOI：
10.1016/s0167-4889(98)00104-9
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Goldstein,RH
中科院分区：
文献类型：
--
作者：
Rishikof,DC;Krupsky,M;Goldstein,RH
前列腺素E2(PGE2)是一种炎症介质，能够调节成纤维细胞的增殖、基质蛋白的产生和A系统氨基酸的转运。系统x−氨基酸的转运受亲电剂和氧气的调节。观察了前列腺素E_2(PGE_2)对人肺成纤维细胞半胱氨酸x-−转运和谷胱甘肽合成的影响。用PGE2预培养成纤维细胞，可使胱氨酸摄取减少42%。动力学研究表明，经前列腺素E_2处理的成纤维细胞中，x-−转运蛋白的Vmax降低了42%，但表观Km没有受到影响。经PGE2处理的成纤维细胞的谷胱甘肽含量最高可降低25%。这些结果表明，−系统对半胱氨酸的转运受前列腺素E_2的调节，提示细胞内半胱氨酸的可获得性有限抑制了谷胱甘肽的合成。
Prostaglandin E2(PGE2) is an inflammatory mediator capable of regulating fibroblast cell proliferation, matrix protein production, and system A amino acid transport. System x−camino acid transport is regulated by electrophilic agents and oxygen. The effect of PGE2on the x−csystem transport of cystine and the synthesis of glutathione by human lung fibroblasts was examined. Preincubation of fibroblast cultures with PGE2decreased cystine uptake by 42%. Kinetic studies revealed a 42% decrease in the Vmaxof the x−csystem transporter in PGE2-treated fibroblasts; however, the apparent Kmwas not affected. The glutathione content of PGE2-treated fibroblasts was decreased by up to 25% of control. These results demonstrate that system x−ctransport of cystine is regulated by PGE2and suggest that the limited availability of intracellular cysteine inhibited glutathione synthesis.