Diagnostic profiling of salivary exosomal microRNAs in oral lichen planus patients
Diagnostic profiling of salivary exosomal microRNAs in oral lichen planus patients
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DOI：
10.1111/odi.12374
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发表时间：
2015-11-01
期刊：
影响因子：
3.8
通讯作者：
Lee, H-J
中科院分区：
文献类型：
--
作者：
Byun, J-S;Hong, S-H;Lee, H-J
目的口腔扁平苔藓是一种慢性炎症性口腔粘膜疾病，其确切病因尚不清楚，需要有效的诊断和治疗策略。唾液中疾病特异性生物标志物的鉴定是一种简单、快速且非侵入性的分子诊断方法。本研究旨在检测唾液外泌体 microRNA (miRNA)，它们可作为诊断和阐明口腔扁平苔藓发病机制的候选者。 对象和方法我们比较了口腔扁平苔藓患者和健康对照者唾液外泌体的 miRNA 谱。将 16 名口腔扁平苔藓患者和 8 名健康对照者的唾液样本分为两组，并使用 miRNA 微阵列分析和 TaqMan 定量 PCR 进行检查。结果鉴定出的 3 个 miRNA（miR-4484、miR-1246 和 miR-1290）得到进一步验证。其中，miR-4484 在口腔扁平苔藓患者的唾液外泌体中显着上调。结论因此，本研究确定了口腔扁平苔藓的潜在 miRNA 生物标志物，并深入了解 miRNA 在口腔炎症疾病发病机制中的功能。
ObjectiveOral lichen planus is a chronic inflammatory oral mucosal disease whose exact cause is unclear and which requires efficient diagnostic and therapeutic strategies. Identification of disease-specific biomarkers in saliva is an easy, quick, and non-invasive approach for molecular diagnosis. This study was designed to examine salivary exosomal microRNAs (miRNAs) that could be candidates for diagnosing and elucidating the pathogenesis of oral lichen planus.Subjects and MethodsWe compared miRNA profiles of salivary exosomes of patients with oral lichen planus with those of healthy controls. Saliva samples from 16 patients with oral lichen planus and eight healthy controls were divided into two sets and examined using miRNA microarray analysis and TaqMan quantitative PCR.ResultsThe three miRNAs identified (miR-4484, miR-1246, and miR-1290) were further validated. Of these, miR-4484 was significantly upregulated in the salivary exosomes of patients with oral lichen planus.ConclusionsThis study thus identifies a potential miRNA biomarker for oral lichen planus and provides insight into the functions of miRNAs in the pathogenesis of oral inflammatory diseases.