Ginsenoside Rg1 Performs Anti-Aging Functions by Suppressing Mitochondrial Pathway-Mediated Apoptosis and Activating Sirtuin 3 (SIRT3)/Superoxide Dismutase 2 (SOD2) Pathway in Sca-1+ HSC/HPC Cells of an Aging Rat Model
Ginsenoside Rg1 Performs Anti-Aging Functions by Suppressing Mitochondrial Pathway-Mediated Apoptosis and Activating Sirtuin 3 (SIRT3)/Superoxide Dismutase 2 (SOD2) Pathway in Sca-1+ HSC/HPC Cells of an Aging Rat Model
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人参皂苷 Rg1 通过抑制衰老大鼠模型 Sca-1 HSC/HPC 细胞中线粒体途径介导的细胞凋亡和激活 Sirtuin 3 (SIRT3)/超氧化物歧化酶 2 (SOD2) 途径来发挥抗衰老功能
DOI：
10.12659/msm.920666
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发表时间：
2020-04-07
影响因子：
3.1
通讯作者：
Ding, Ji-Chao
中科院分区：
文献类型：
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作者：
Zhou, Yue;Wang, Yan-Ping;Ding, Ji-Chao
背景：衰老的特点是代谢和生理过程逐渐恶化。本研究评估了人参皂苷Rg1（Rg1）对HSCs/HPCs衰老的拮抗作用和机制。 材料/方法：将50只雄性Sprague-Dawley（SD）大鼠治疗并分为以下组：对照组（n=10）、模型组（n=10，用D-半乳糖处理作为衰老模型）、Rg1对照组（n=10）、Rg1治疗组 (n=10) 和 Rg1 预防 (n=10)。通过皮下注射D-gal建立衰老大鼠模型。使用SA-β-Gal染色对HSC/HPC细胞进行染色。使用流式细胞术检测 HSC/HPC 细胞。进行了一些修改后的 CFU 混合测定。使用 qRT-PCR 检查裂解的 caspase-3、B 细胞淋巴瘤-2 (Bcl-2) 和 Bcl-2 相关 X 蛋白 (Bax)。使用蛋白质印迹法和 qRT-PCR 测定 Sirtuin 3 (SIRT3) 和超氧化物歧化酶 2 (SOD2) 的表达。 结果：与 D-gal 模型相比，Rg1（治疗组和预防组）显着降低 Sca-1(+) HSC/HPC 细胞中 SA-β-Gal 阳性染色（p
Background: Aging is characterized by progressive deterioration in metabolic and physiological process. The present research assessed the antagonistic effects and mechanisms of Ginsenoside Rg1 (Rg1) on aging of HSCs/HPCs.Material/Methods: Fifty male Sprague-Dawley (SD) rats were treated and divided into the following groups: Control (n=10), Model (n=10, treated with D-galactose, as aging model), Rg1 Control (n=10), Rg1 treatment (n=10), and Rg1 prevention (n=10). An aging rat model was established by subcutaneous injection with D-gal. HSC/HPC cells were stained using SA-beta-Gal staining. HSC/HPC cells were examined using flow cytometry assay. CFU-mix assay, with a few modifications, was performed. Cleaved caspase-3, B-cell lymphoma-2 (Bcl-2), and Bcl-2-associated X protein (Bax) were examined using qRT-PCR. Sirtuin 3 (SIRT3) and superoxide dismutase 2 (SOD2) expression was determined using Western blot assay and qRT-PCR.Results: Rg1 (treatment and prevention group) significantly decreased SA-beta-Gal-positive staining in Sca-1(+) HSC/HPC cells compared to that of the D-gal model (p