The study of the three‐dimensional structural relationships in connective tissues by high voltage electron microscopy
The study of the three‐dimensional structural relationships in connective tissues by high voltage electron microscopy
复制标题
高压电镜研究结缔组织三维结构关系
作者：
A. Glauert;C. Mayo
在AEI EM7高压电子显微镜下，在1 MV下对0.5-1.0 μm厚的切片进行立体电子显微镜检查，提供了结缔组织结构关系的三维信息。用醋酸铀酰块染色和用柠檬酸铅长时间染色后获得充分的对比。当省略醋酸铀酰或铅染色时，观察到选择性染色。初步结果表明，在分泌胶原蛋白的软骨细胞中可能存在粗内质网池池与质膜融合的区域。观察结果还表明，钙化区域的基质囊泡是由成骨细胞的长细胞质过程通过“挤压”机制形成的，骨晶只有在囊泡与周围细胞完全分离后才会在囊泡内形成。结论是，高压电子显微镜能够为理解与特定生化事件相关的三维结构关系做出重大贡献。
An examination of stereo‐electron micrographs taken at 1 MV in an AEI EM7 high voltage electron microscope of sections 0.5–1.0 μm thick has provided three‐dimensional information of the structural relationships in connective tissues. Sufficient contrast is obtained after block staining with uranyl acetate and with prolonged staining of the sections with lead citrate. Selective staining was observed when either the uranyl acetate or lead staining was omitted. Preliminary results indicate that there may be regions of fusion of the cisternae of the rough endoplasmic reticulum and the plasma membrane in chondrocytes that are secreting collagen. The observations also suggest that matrix vesicles in regions of calcification are formed from long cytoplasmic processes of osteoblasts by a ‘pinching off’ mechanism and that bone crystallites form within the vesicles only after the vesicles have completely separated from the surrounding cells. It is concluded that the high voltage electron microscope is capable of making a significant contribution to the understanding of the three‐dimensional structural relationships associated with specific biochemical events.