Silver nanoparticles enhance thrombus formation through increased platelet aggregation and procoagulant activity
Silver nanoparticles enhance thrombus formation through increased platelet aggregation and procoagulant activity
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DOI：
10.3109/17435390.2010.506250
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发表时间：
2011-06-01
期刊：
影响因子：
5
通讯作者：
Chung, Jin-Ho
中科院分区：
文献类型：
--
作者：
Jun, Eun-Ah;Lim, Kyung-Min;Chung, Jin-Ho
尽管银纳米颗粒（纳米银）被广泛使用，但其毒性仍然知之甚少。在本报告中，纳米银诱导血小板聚集和促凝剂激活的增加，这是血栓性疾病的关键因素。在新鲜分离的人血小板中，纳米银诱导血小板聚集和促凝剂激活，这可以通过增加磷脂酰丝氨酸暴露和凝血酶的产生来证明。有趣的是，亚阈值水平的凝血酶显著增强了纳米Ag诱导的血小板活化，这表明纳米Ag在活化血小板中的促血栓作用可能进一步增强。纳米银增加细胞内钙介导的血小板活化和p -选择素的表达，并促进血清素的释放。与体外实验结果一致，体内暴露于纳米银（0.05—0.1 mg/kg静脉滴注或5—10 mg/kg气管内滴注）可促进大鼠体内静脉血栓形成、血小板聚集和磷脂酰丝氨酸外化，这表明纳米银确实通过血小板活化促进血栓形成。
Despite the wide use of silver nanoparticles (nano Ag), its toxicity still remains poorly understood. In this report, nano Ag induced an increase in platelet aggregation and procoagulant activation which are the key contributors to thrombotic diseases. In freshly isolated human platelets, nano Ag induced platelet aggregation and procoagulant activation evident by increased phosphatidylserine exposure and thrombin generation. Interestingly, the sub-threshold level of thrombin enhanced nano Ag-induced platelet activation significantly indicating that the prothrombotic effects of nano Ag might be further potentiated in activated platelets. An increase in intracellular calcium mediated nano Ag induced platelet activation and P-selectin expression, and serotonin release was also enhanced by nano Ag. Consistent with the in vitro results, exposure to nano Ag (0.05--0.1 mg/kg i.v. or 5--10 mg/kg intratracheal instillation) in vivo enhanced venous thrombus formation, platelet aggregation, and phosphatidylserine externalization ex vivo in rats suggesting that nano Ag, indeed, does enhance thrombus formation through platelet activation.