Effect of Ovary Storage on Development of Bovine Oocytes after Intracytoplasmic Sperm Injection, Parthenogenetic Activation, or Somatic Cell Nuclear Transfer
Effect of Ovary Storage on Development of Bovine Oocytes after Intracytoplasmic Sperm Injection, Parthenogenetic Activation, or Somatic Cell Nuclear Transfer
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卵巢储存对胞浆内单精子注射、孤雌激活或体细胞核移植后牛卵母细胞发育的影响
DOI：
10.1274/jmor.24.114
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发表时间：
2007
期刊：
影响因子：
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通讯作者：
Seiya Takahashi
中科院分区：
文献类型：
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作者：
K. Matsukawa;S. Akagi;N. Adachi;M. Kubo;M. Hirako;S. Watanabe;Seiya Takahashi
本研究旨在探讨卵母细胞胞浆内单精子注射(ICSI)、孤雌激活或体细胞核移植(SCNT)后卵巢保存对牛卵母细胞发育的影响。卵巢在15℃的PBS中保存2~6h(对照组)或26~30h(保存组)可获得卵母细胞，保存组卵母细胞成熟率(67%)显著低于对照组(78%)。保存组卵母细胞的退化率(24%)显著高于对照组(2%)。ICSI和来自保存卵巢的孤雌激活卵母细胞的囊胚期发育率显著低于对照组(ICSI 8%比24%，孤雌激活15%比31%)。然而，卵丘细胞来源的SCNT胚胎的囊胚率在两组之间没有差异(38%比38%)。卵巢保存时间的延长不会降低SCNT胚胎的妊娠率，两组克隆牛的发育率分别为21%和21%。综上所述，卵巢在15℃下保存26~30h可以降低牛卵母细胞ICSI或孤雌激活后的成熟率和体外发育率，但不会降低SCNT胚胎移植后的囊胚率和存活率。
ABSTRACT The purpose of this study was to examine the effect of ovary storage on the development of bovine oocytes after intracytoplasmic sperm injection (ICSI), parthenogenetic activation, or somatic cell nuclear transfer (SCNT). Oocytes were obtained from ovaries stored in PBS for 2 to 6 h (control group) or 26 to 30 h (stored group) at 15°C. The maturation rate of the oocytes was significantly lower in the stored group (67%) than in the control group (78%). The degeneration rate of the oocytes was significantly higher in the stored group (24%) than in the control group (2%). ICSI and parthenogenetic oocytes from stored ovaries had a significantly decreased development to the blastocyst stage compared with the control (ICSI 8% vs. 24%, parthenogenetic activation 15% vs. 31%). However, the development rate to blastocysts of SCNT embryos derived from cumulus cells was not different between the two groups (38% vs. 38%). Also, the storage period of ovaries did not decrease the pregnancy rate of SCNT embryos, and cloned calves were produced in both groups with the same efficiency (21% vs. 21%). In summary, ovary storage at 15°C for 26 to 30 h reduced the maturation rate and in vitro development rate of bovine oocytes after ICSI or parthenogenetic activation, but did not decrease the blastocyst formation rate or survival rate after embryo transfer in SCNT.
Horiuchi, T.、Emuta, C.、Yamauchi, Y.、Oikawa, T.、Numabe, T.、Yanagimachi, R.：“牛卵母细胞胞浆内精子注射后正常犊牛的出生：方法学方法”57。・3.1013-1024（2002）
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牛卵巢低温保存对体外受精、孤雌激活或体细胞核移植后卵母细胞成熟率和发育潜力的影响
DOI：
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发表时间：
2004
期刊：
Animal Reproduction Science 84
影响因子：
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作者：
Matsushita S.;et al.
通讯作者：
et al.