Hin-mediated DNA knotting and recombining promote replicon dysfunction and mutation
Hin-mediated DNA knotting and recombining promote replicon dysfunction and mutation
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DOI：
10.1186/1471-2199-8-44
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发表时间：
2007-05-25
影响因子：
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通讯作者：
Zechiedrich, Lynn
中科院分区：
文献类型：
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作者：
Deibler, Richard W.;Mann, Jennifer K.;Zechiedrich, Lynn
背景：遗传密码使细胞陷入困境。DNA必须足够长，以编码有机体的复杂性，但又必须足够薄和灵活，以适应细胞。这些特性的组合极大地促进了DNA碰撞，这可以打结并驱动DNA的重组。尽管公认的倾向，细胞DNA碰撞和反应本身，它还没有建立什么生理后果是.Results：在这里，我们分析了重组和打结质粒在大肠杆菌中的影响。大肠杆菌中使用Hin位点特异性重组系统。我们发现，Hin介导的DNA打结和重组（一）促进复制子的损失，通过阻断DNA复制;（二）阻止基因转录;和（三）导致基因重排的速率高出3至4个数量级的速率为unknotted，unrecombined plasmid.Conclusion：这些结果表明，DNA反应导致重组和打结的DNA是潜在的毒性，可能有助于推动遗传进化。
Background: The genetic code imposes a dilemma for cells. The DNA must be long enough to encode for the complexity of an organism, yet thin and flexible enough to fit within the cell. The combination of these properties greatly favors DNA collisions, which can knot and drive recombination of the DNA. Despite the well-accepted propensity of cellular DNA to collide and react with itself, it has not been established what the physiological consequences are.Results: Here we analyze the effects of recombined and knotted plasmids in E. coli using the Hin site-specific recombination system. We show that Hin-mediated DNA knotting and recombination (i) promote replicon loss by blocking DNA replication; (ii) block gene transcription; and (iii) cause genetic rearrangements at a rate three to four orders of magnitude higher than the rate for an unknotted, unrecombined plasmid.Conclusion: These results show that DNA reactivity leading to recombined and knotted DNA is potentially toxic and may help drive genetic evolution.