Nontypeable Haemophilus influenzae in the lower respiratory tract of patients with chronic bronchitis
Nontypeable Haemophilus influenzae in the lower respiratory tract of patients with chronic bronchitis
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DOI：
10.1164/ajrccm.164.11.2104093
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发表时间：
2001-12-01
影响因子：
24.7
通讯作者：
Greenberg, SB
中科院分区：
文献类型：
--
作者：
Bandi, V;Apicella, MA;Greenberg, SB
在健康成人以及临床稳定和急性慢性支气管炎（CB）患者中测定了不可分型流感嗜血杆菌（NTHi）在下呼吸道的定植和细胞内定位频率。 NTHi 在 23 名稳定 CB 患者中的 6 名 (26%) 和 15 名呼吸恶化的 CB 患者中的 1 名 (7%) 中从支气管冲洗液或支气管刷标本中回收。从接受择期手术麻醉的健康成年人的下消化道标本中未回收到 NTHi（26 份中的 0 份）。 NTHi 菌株的分子分型显示，9 名稳定 CB 患者中的 5 名在同时收集的上呼吸道和支气管冲洗/刷洗标本中存在不同菌株。四名稳定的 CB 患者在重复支气管镜检查时恢复了不同的菌株。这些结果表明，具有多种 NTHi 菌株的稳定 CB 患者的下呼吸道频繁定植。还通过原位杂交和免疫荧光显微镜检查了支气管活检中的细胞内 NTHi。在 7 名健康成人中的 0 名、24 名临床稳定 CB 患者中的 8 名以及 15 名急性病 CB 患者中的 13 名中发现了细胞内 NTHi。这一观察结果表明 NTHi 的细胞内感染在 CB 恶化的发病机制中发挥着作用。
The frequency of colonization and intracellular localization of nontypeable Haemophilus influenzae (NTHi) in the lower respiratory tract was determined in healthy adults and in clinically stable and acutely Ill chronic bronchitis (CB) patients. NTHi was recovered from bronchial wash or bronchial brush specimens in 6 of 23 (26%) stable CB patients and in 1 of 15 (7%) CB patients with a respiratory exacerbation. No NTHi (0 of 26) was recovered from lower tract specimens of healthy adults undergoing anesthesia for elective surgery. Molecular typing of NTHi strains revealed that five of nine patients with stable CB had different strains in upper respiratory tract and bronchial wash/brush specimens collected simultaneously. Four stable patients with CB had different strains recovered on repeat bronchoscopy. These results demonstrate the frequent colonization of the lower airways of stable CB patients with multiple strains of NTHi Bronchial biopsies also were examined for intracellular NTHi by in situ hybridization and immunofluorescence microscopy. Intracellular NTHi were found in 0 of 7 healthy adults, 8 of 24 patients with clinically stable CB, and 13 of 15 acutely ill CB patients. This observation suggests a role for intracellular infection by NTHi in the pathogenesis of exacerbations of CB.