Photoacoustic monitoring of tumor and normal tissue response to radiation.
Photoacoustic monitoring of tumor and normal tissue response to radiation.
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DOI：
10.1038/srep21237
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发表时间：
2016-02-17
影响因子：
4.6
通讯作者：
Seshadri M
中科院分区：
文献类型：
--
作者：
Rich LJ;Seshadri M
低氧是公认的影响放射治疗(RT)疗效的肿瘤特征。光声成像(PAI)是一种利用血红蛋白的光学特性来提供组织氧合信息的相对较新的成像技术。在本研究中，在患者来源的异种移植头颈癌模型中，比较了基于PAI的肿瘤氧饱和度(%SO2)测量与氧增强磁共振成像测量的纵向松驰率(r1 = 1/t1)和体外组织学。PAI用于评估放疗和化疗后早期(24 h)%SO2的变化，以及放疗后唾液腺血流动力学的变化。观察到吸氧后肿瘤%SO2和R1显著增加。PAI、MRI与组织学具有良好的空间相关性。PAI检测到放疗和CRT后早期%SO2的升高与显著的肿瘤生长抑制有关。放疗24小时后，PAI还检测到小鼠唾液腺组织对味觉刺激的血流动力学反应丧失，提示辐射诱导的血管损伤。我们的观察结果说明了PAI在检测头颈部肿瘤和正常组织对放射治疗的血流动力学反应方面的有效性。
Hypoxia is a recognized characteristic of tumors that influences efficacy of radiotherapy (RT). Photoacoustic imaging (PAI) is a relatively new imaging technique that exploits the optical characteristics of hemoglobin to provide information on tissue oxygenation. In the present study, PAI based measures of tumor oxygen saturation (%sO2) were compared to oxygen-enhanced magnetic resonance imaging (MRI) measurements of longitudinal relaxation rate (R1 = 1/T1) and ex-vivo histology in patient derived xenograft (PDX) models of head and neck cancer. PAI was utilized to assess early changes (24 h) in %sO2 following RT and chemoRT (CRT) and to assess changes in salivary gland hemodynamics following radiation. A significant increase in tumor %sO2 and R1 was observed following oxygen inhalation. Good spatial correlation was observed between PAI, MRI and histology. An early increase in %sO2 after RT and CRT detected by PAI was associated with significant tumor growth inhibition. Twenty four hours after RT, PAI also detected loss of hemodynamic response to gustatory stimulation in murine salivary gland tissue suggestive of radiation-induced vascular damage. Our observations illustrate the utility of PAI in detecting tumor and normal tissue hemodynamic response to radiation in head and neck cancers.