GLUT3 protein and mRNA in autopsy muscle specimens
GLUT3 protein and mRNA in autopsy muscle specimens
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DOI：
10.1016/s0026-0495(99)90222-6
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发表时间：
1999-07-01
影响因子：
9.8
通讯作者：
Jiang, J
中科院分区：
文献类型：
--
作者：
Stuart, CA;Wen, G;Jiang, J
GLUT3在大鼠肌肉中表达，但这种葡萄糖转运蛋白在成人骨骼肌中尚未被鉴定。我们量化了人骨骼肌在室温和4℃下mRNA和蛋白质消失的速度。在20℃下1小时和4℃下2小时，免疫检测到的GLUT3蛋白有50%消失。GLUT3的mRNA在20℃下2.2小时和4℃下24小时下降50%。而β-肌球蛋白在20摄氏度时消失0.8至2.1小时，在4摄氏度时消失5.0至16.6小时。以前得出的结论是，GLUT3在人类肌肉中不表达，可能是因为研究前标本中与GLUT3蛋白降解有关的人工制品。由于蛋白质和mRNA的快速降解，肌肉的尸检样本必须在死亡后6小时内获得，即使在那时，蛋白质和mRNA的数据也可能大大低估它们在新鲜肌肉中的表达。以前发表的一些关于尸检标本的结论和建议不够严格，不足以持续地产生有用的蛋白质和mRNA。版权所有(C)1999，由W.B.Saunders公司提供。
GLUT3 is expressed in rat muscle, but this glucose transporter protein has not been identified previously in adult human skeletal muscle. We quantified the rapidity of disappearance of mRNA and protein from human skeletal muscle at room temperature and at 4 degrees C. Fifty percent of the immunologically detectable GLUT3 protein disappeared by 1 hour at 20 degrees C and by 2 hours at 4 degrees C. mRNA for GLUT3 was decreased 50% by 2.2 hours at 20 degrees C and by 24 hours at 4 degrees C. Half of the measurable mRNAs for GLUT4, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), alpha-actin, and beta-myosin disappeared by 0.8 to 2.1 hours at 20 degrees C and by 5.0 to 16.6 hours at 4 degrees C. Previous conclusions that GLUT3 is not expressed in human muscle were likely drawn because of artifacts related to degradation of GLUT3 protein in the specimens prior to study. Because of the rapid degradation of protein and mRNA, autopsy specimens of muscle must be obtained within 6 hours of death, and even then, protein and mRNA data will likely dramatically underestimate their expression in fresh muscle. Some previously published conclusions and recommendations regarding autopsy specimens are not stringent enough to consistently yield useful protein and mRNA. Copyright (C) 1999 by W.B. Saunders Company.